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Assessment for evidence of apoptosis of myenteric ganglion cells at the transition zone in Hirschsprung's Disease and the developing large intestine

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dc.contributor.supervisor Grynspan, David (Pathology) en
dc.contributor.author Carter, Terri Anne
dc.date.accessioned 2009-08-20T21:25:01Z
dc.date.available 2009-08-20T21:25:01Z
dc.date.issued 2009-08-20T21:25:01Z
dc.identifier.uri http://hdl.handle.net/1993/3173
dc.description.abstract Introduction: Hirschsprung’s Disease (HD) is the congenital absence of ganglion cells (GCs) within the distal intestine. Our objectives are to determine if apoptosis of myenteric GCs occurs during human development and to determine if myenteric GC apoptosis or injury contributes to HD. Materials and Methods: Apoptosis of myenteric GCs was assessed in archived fetal intestinal tissue (n = 4; 15-41 weeks gestational age) and in HD at the transition zone (TZ) (n = 6) using anti-cleaved caspase-3. Immunohistochemistry for GFAP, CD68, HLA-DR and APP was used to assess the presence of enteric reactive changes. Results: No activated caspase-3 expression was present in the myenteric GCs of the developing human intestine or the TZ of HD. No significant increase in GFAP, CD68, HLA-DR or APP expression was present. Conclusions: Apoptosis does not appear to occur during the development of the human myenteric plexus or, in conjunction with GC injury, in HD. en
dc.format.extent 1312694 bytes
dc.format.mimetype application/pdf
dc.language.iso en_US
dc.rights info:eu-repo/semantics/openAccess
dc.subject Pathology en
dc.subject Human Development en
dc.subject Hirschsprung's Disease en
dc.subject Gastrointestinal tract en
dc.subject Apoptosis en
dc.subject Ganglion cells en
dc.title Assessment for evidence of apoptosis of myenteric ganglion cells at the transition zone in Hirschsprung's Disease and the developing large intestine en
dc.type info:eu-repo/semantics/masterThesis
dc.type master thesis en_US
dc.degree.discipline Pathology en
dc.contributor.examiningcommittee Eisenstat, David (Director, Advanced Degrees in Medicine Program, Faculty of Medicine; Director, Neuro-Oncology, CancerCare Manitoba; Departments of Pediatrics & Child Health, Human Anatomy & Cell Science, Ophthalmology, and Biochemistry & Medical Genetics; Senior Investigator, Manitoba Institute of Cell Biology; Scientist, Manitoba Institute of Child Health) Del Bigio, Marc (Canada Research Chair in Developmental Neuropathology; Department of Pathology, Human Anatomy, and Pediatrics & Child Health) en
dc.degree.level Master of Science (M.Sc.) en
dc.description.note October 2009 en


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