Metagenomic analysis of rumen microbial population in dairy heifers fed a high grain diet supplemented with dicarboxylic acids or polyphenols

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dc.contributor.author De Nardi, Roberta
dc.contributor.author Marchesini, Giorgio
dc.contributor.author Li, Shucong
dc.contributor.author Khafipour, Ehsan
dc.contributor.author Plaizier, Kees J C
dc.contributor.author Gianesella, Matteo
dc.contributor.author Ricci, Rebecca
dc.contributor.author Andrighetto, Igino
dc.contributor.author Segato, Severino
dc.date.accessioned 2016-02-22T16:24:28Z
dc.date.available 2016-02-22T16:24:28Z
dc.date.issued 2016-02-19
dc.identifier.citation BMC Veterinary Research. 2016 Feb 19;12(1):29
dc.identifier.uri http://dx.doi.org/10.1186/s12917-016-0653-4
dc.identifier.uri http://hdl.handle.net/1993/31135
dc.description.abstract Abstract Background The aim of this study was to investigate the effects of two feed supplements on rumen bacterial communities of heifers fed a high grain diet. Six Holstein-Friesian heifers received one of the following dietary treatments according to a Latin square design: no supplement (control, C), 60 g/day of fumarate-malate (organic acid, O) and 100 g/day of polyphenol-essential oil (P). Rumen fluid was analyzed to assess the microbial population using Illumina sequencing and quantitative real time PCR. Results The P treatment had the highest number of observed species (P < 0.10), Chao1 index (P < 0.05), abundance based coverage estimated (ACE) (P < 0.05), and Fisher’s alpha diversity (P < 0.10). The O treatment had intermediate values between C and P treatments with the exception of the Chao1 index. The PCoA with unweighted Unifrac distance showed a separation among dietary treatments (P = 0.09), above all between the C and P (P = 0.05). The O and P treatments showed a significant increase of the family Christenenellaceae and a decline of Prevotella brevis compared to C. Additionally, the P treatment enhanced the abundance of many taxa belonging to Bacteroidetes, Firmicutes and Tenericutes phyla due to a potential antimicrobial activity of flavonoids that increased competition among bacteria. Conclusions Organic acid and polyphenols significantly modified rumen bacterial populations during high-grain feeding in dairy heifers. In particular the polyphenol treatment increased the richness and diversity of rumen microbiota, which are usually high in conditions of physiological rumen pH and rumen function.
dc.title Metagenomic analysis of rumen microbial population in dairy heifers fed a high grain diet supplemented with dicarboxylic acids or polyphenols
dc.type Journal Article
dc.language.rfc3066 en
dc.rights.holder De Nardi et al.
dc.date.updated 2016-02-19T17:02:41Z

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