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Analysis of genetic diversity and expression of genes involved in fatty acid composition in flax (Linum usitatissimum L.) and comparative genomic analysis of their loci

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dc.contributor.supervisor Cloutier, Sylvie (Plant Science) en_US
dc.contributor.author Thambugala, Dinushika
dc.date.accessioned 2015-08-13T16:03:32Z
dc.date.available 2015-08-13T16:03:32Z
dc.date.issued 2013 en_US
dc.identifier.citation Thambugala D, Duguid S, Loewen E, Rowland G, Booker H, You FM, Cloutier S (2013) Genetic variation of six desaturase genes in flax and their impact on fatty acid composition. Theor Appl Genet 126:2627-2641 en_US
dc.identifier.uri http://hdl.handle.net/1993/30665
dc.description.abstract Flax (Linum usitatissimum L.) is one of the richest plant sources of omega-3 fatty acids praised for their health benefits. In this study, the extent of the genetic variability for genes encoding stearoyl-ACP desaturase (SAD), fatty acid desaturase 2 (FAD2) and 3 (FAD3) was determined by sequencing the six paralogous genes from 120 flax accessions representing a broad range of germplasm including some EMS mutant lines. A total of 6 alleles for sad1 and sad2, 21 for fad2a, 5 for fad2b, 15 for fad3a and 18 for fad3b were identified. Deduced amino acid sequences of the alleles predicted 4, 2, 3, 4, 6, and 7 isoforms, respectively. Allele frequencies varied greatly across genes. Fad3a, with 110 SNPs and 19 indels, and fad3b, with 50 SNPs and 5 indels, showed the highest levels of genetic variation. While most of the SNPs and all the indels were silent mutations, both genes carried non-sense SNP mutations resulting in premature stop codons, a feature not observed in sad and fad2 genes. Some alleles and isoforms discovered in induced mutant lines were absent in the natural germplasm. Correlation of these genotypic data with fatty acid composition data of 120 flax accessions phenotyped in six field experiments revealed statistically significant correlations of some of the SAD and FAD isoforms on fatty acid composition, oil content and iodine value. The novel allelic variants and isoforms identified for the six desaturases will be a resource for the development of oilseed flax with unique and useful fatty acid profiles. en_US
dc.publisher Theoretical and Applied Genetics en_US
dc.subject Flax en_US
dc.subject Fatty acids en_US
dc.subject Fatty acid desaturase en_US
dc.title Analysis of genetic diversity and expression of genes involved in fatty acid composition in flax (Linum usitatissimum L.) and comparative genomic analysis of their loci en_US
dc.degree.discipline Plant Science en_US
dc.contributor.examiningcommittee Stasolla, Claudio (Plant Science) Li, Genyi (Plant Science) Piercey-Normore, Michele (Biological Sciences) Qiu, Xiao (Food and Bioproduct Sciences, University of Saskatchewan) en_US
dc.degree.level Doctor of Philosophy (Ph.D.) en_US
dc.description.note October 2015 en_US


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