Home

Processing of lignocellulosics feedstocks for biofuels and co-products via consolidated bioprocessing with the thermophilic bacterium, Clostridium thermocellum strain DSMZ 1237

Show simple item record

dc.contributor.supervisor Levin, David (Biosystems Engineering) Cicek, Nazim (Biosystems Engineering) en_US
dc.contributor.author Agbor, Valery
dc.date.accessioned 2015-07-30T19:44:13Z
dc.date.available 2015-07-30T19:44:13Z
dc.date.issued 2011 en_US
dc.date.issued 2014 en_US
dc.date.issued 2014 en_US
dc.date.issued 2011 en_US
dc.identifier.citation Agbor, VB, Cicek N, Sparling R, Berlin A, Levin DB. 2011. Biomass pretreatment: fundamentals toward application. Biotechnology advances 29, no. 6, 675-685; en_US
dc.identifier.citation Levin DB, Agbor VB, Carare C, Cicek N, Sparling R. 2014. Biomass Pretreatment for Consolidated Bioprocessing (CBP). Chapter 47, In, “Advances in Biorefineries: Biomass and waste supply chain exploitation”, KW Waldron (Ed.). Woodhead Publishing, Cambridge, UK. ISBN-13: 978 0 85709 521 3. en_US
dc.identifier.citation Agbor V, Zurzolo F, Blunt W, Dartiailh C, Cicek N, Sparling R, Berlin A, Levin DB. 2014. Single-step fermentation of hemp residues for hydrogen and ethanol production. Biomass and Bioenergy, 64, 62-69. en_US
dc.identifier.citation Agbor V, Blunt W, Cicek N, Sparling R, Berlin A, Levin DB. 2011. Processing of celluylosic feedstocks for ethanol and hydrogen production. Journal of Science and Technology for Forest Products and Processes, 1, 54-61 en_US
dc.identifier.uri http://hdl.handle.net/1993/30647
dc.description.abstract Processing of lignocellulosic biomass for transportation fuels and other biocommodities in integrated biorefineries has been proposed as the future for emerging sustainable economies. Currently bioprocessing strategies are all multi-step processes involving extensive physicochemical pretreatments and costly amounts of exogenous enzyme addition. Consolidated bioprocessing (CBP), or direct microbial conversion, is a strategy that combines all the stages of production into one step, thus avoiding the use of expensive pretreatments and exogenous enzymes that reduce the economic viability of the products produced. With a growing trend towards increased consolidation, most of the reported work on CBP has been conducted with soluble sugars or commercial reagent grade cellulose. For CBP to become practical fermentative guidelines with native feedstocks and purified cellulose need to be delineated through specific substrate characterization as it relates to possible industrial fermentation. By carefully reviewing the fundamentals of biomass pretreatments for CBP, a comparative assessment of the fermentability of non-food agricultural residue and processed biomass was conducted with Clostridium thermocellum DSMZ 1237. Cell growth, and both gaseous and liquid fermentation end-product profiles of C. thermocellum as a CBP processing candidate was characterised. Batch fermentation experiments to investigate the effect of cellulose content, pretreatment, and substrate concentration, revealed that higher yields were correlated with higher cellulose content. Pretreatment of native substrates that increased access of the bacterial cells and enzymes to cellulose chains in the biomass substrate were key parameters that determined the overall bioconversion of a given feedstock to end-products. The contribution of amorphous cellulose (CAC) in different biomass substrates subjected to the same pretreatment conditions was identified as a novel factor that contributed to differences in bioconversion and end-product synthesis patterns. Although the overall yield of end products was low following bioaugmentation with exogenous glycosyl hydrolases from free-enzyme systems and cellulosome extracts. Treatment of biomass substrates with glycosyl hydrolase enzymes was observed to increase the rate of bioconversion of native feedstocks in biphasic manner during fermentation with C. thermocellum. A “quotient of accessibility” was identified as a feedstock agnostic guideline for biomass digestibility. en_US
dc.publisher Biotechnology Advances / ELSEVIER en_US
dc.publisher Woodhead Publishing, Cambridge, UK / ELSEVIER en_US
dc.publisher Biomass and Bioenergy / ELSEVIER en_US
dc.publisher Journal of Science and Technology for Forest Products and Processes en_US
dc.subject Biomass en_US
dc.subject Consolidated en_US
dc.subject Bioprocessing en_US
dc.subject Biofuels en_US
dc.subject Fermentation en_US
dc.subject pretreatment en_US
dc.title Processing of lignocellulosics feedstocks for biofuels and co-products via consolidated bioprocessing with the thermophilic bacterium, Clostridium thermocellum strain DSMZ 1237 en_US
dc.degree.discipline Biosystems Engineering en_US
dc.contributor.examiningcommittee Butler, Michael (Microbiology) Chen, Ying (Biosystems Engineering) Berlin, Alex (Biosystems Engineering) Cheng, Jay J. (Biological and Agricultural Engineering, North Carolina State University) en_US
dc.degree.level Doctor of Philosophy (Ph.D.) en_US
dc.description.note October 2015 en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record

View Statistics