Modulation of ICa-L by alpha(1)-adrenergic stimulation in rat ventricular myocytes
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Date
2005-11-30
Authors
Zhang, ST
Lin, JJ
Hirano, YJ
Hiraoka, M
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Abstract
We found when L-type calcium current (ICa-L) was recorded with the perforated patch-clamp method in rat ventricular myocytes that bath application of phenylephrine (with propranolol) evoked a biphasic response characterized by an initial transient suppression followed by a sustained potentiation. The transient suppression occurred 30-60 s after phenylephrine perfusion and reached peak inhibition at approximately 2 min. The biphasic modulation of ICa-L was also elicited by methoxamine, and the effects of phenylephrine were blocked by prazosin, indicating that the responses were mediated through alpha(1)-adrenoceptors. Pretreatment of cells with H7 (100 mu mol/L), a broad-spectrum protein kinase inhibitor that inhibits both protein kinase C and A, eliminated potentiation but did not affect transient suppression. The transient suppression occurred concurrently with the acceleration of the fast component of ICa-L inactivation. Depletion of intracellular Ca2+ stores by ryanodine plus caffeine or thapsigargin eliminated the transient suppression. When ICa-L was recorded with whole-cell patch-clamp and with 0.05 mmol/L EGTA in the pipette solution to allow intracellular Ca2+ to fluctuate, phenylephrine evoked a transient suppression as in the perforated patch recordings. Heparin, a specific blocker of IP3 (inositol 1,4,5-trisphosphate) receptors, eliminated the phenylephrine-induced transient suppression of ICa-L when added to the pipette solution. Intensive chelation of intracellular Ca2+ by 5 mmol/L BAPTA (1,2-bis(2aminophenoxy)ethane-N,N,N',N'-tetraacetic acid) in the pipette solution also eliminated the phenylephrine-induced transient suppression of ICa-L We conclude that transient increase in the concentration of intracellular calcium ([Ca2+](i)) caused by Ca2+ release from intracellular stores underlies the transient suppression of ICa-L, whereas the potentiation of ICa-L is a result of activation of protein kinases.
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Keywords
Ca2(+) mobilization, IP3, Ca2+-induced inactivation of Ca2+ current, perforated patch-clamp, PROTEIN-KINASE-C, CARDIAC PURKINJE-FIBERS, SMOOTH-MUSCLE CELLS, CALCIUM-CHANNELS, ALPHA-1-ADRENOCEPTOR STIMULATION, SIGNAL-TRANSDUCTION, MAMMALIAN HEART, ANGIOTENSIN-II, CA2+ CURRENT, K+ CURRENT
Citation
0008-4212; CAN J PHYSIOL PHARMACOL, NOV 2005, vol. 83, no. 11, p.1015 to 1024.