Human growth factor receptor bound 14 binds the activated insulin receptor and alters the insulin-stimulated tyrosine phosphorylation levels of multiple proteins

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dc.contributor.author Hemming, R
dc.contributor.author Agatep, R
dc.contributor.author Badiani, K
dc.contributor.author Wyant, K
dc.contributor.author Arthur, G
dc.contributor.author Gietz, RD
dc.contributor.author Triggs-Raine, B
dc.date.accessioned 2007-10-05T16:08:15Z
dc.date.available 2007-10-05T16:08:15Z
dc.date.issued 2001-02-28
dc.identifier.citation 0829-8211; BIOCHEM CELL BIOL, FEB 2001, vol. 79, no. 1, p.21 to 32. en
dc.identifier.uri http://hdl.handle.net/1993/2898
dc.description.abstract To identify proteins interacting in the insulin-signaling pathway that might define new pathways or regulate existing ones, we have employed the yeast two-hybrid system. In a two-hybrid screen of a human liver cDNA library, we identified the human growth factor receptor bound 14 (hGrb14) adaptor protein as a partner of the activated insulin receptor. Additional analysis of the insulin receptor - hGrb14 interaction in the yeast two-hybrid system revealed that the SH2 domain of hGrb14 was not the sole region involved in binding the activated insulin receptor. The insulin-stimulated interaction between hGrb14 and the insulin receptor was also observed in different mammalian cultured cell lines. This association was detected at 1 min of insulin stimulation and was maximal at 10 nM and greater concentrations of insulin. Chinese hamster ovary cells stably expressing the insulin receptor (CHO-IR) and hGrb14 were used to examine the effects of hGrb14 overexpression on insulin-stimulated tyrosine phosphorylation of proteins; in general, increasing levels of hGrb14 expression resulted in a reduction in tyrosine phosphorylation. This decrease was demonstrated for the specific proteins src homology-containing and collagen-related protein (Shc), insulin receptor substrate-1 (IRS-1), and Downstream of tyrosine Kinase (Dok). The broad effects of hGrb14 overexpression on insulin-stimulated tyrosine phosphorylation suggest that it acts early in the insulin-signaling pathway. en
dc.format.extent 1517037 bytes
dc.format.mimetype application/pdf
dc.language.iso en_US
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dc.subject insulin signaling en
dc.subject growth factor receptor bound 14 en
dc.subject Grb14 en
dc.subject adaptor protein en
dc.subject insulin receptor en
dc.subject FACTOR-I RECEPTOR en
dc.subject SRC HOMOLOGY-2 DOMAIN en
dc.subject SH2 DOMAIN en
dc.subject ESCHERICHIA-COLI en
dc.subject 2-HYBRID SYSTEM en
dc.subject ADAPTER PROTEIN en
dc.subject KINASE DOMAIN en
dc.subject GRB-IR en
dc.title Human growth factor receptor bound 14 binds the activated insulin receptor and alters the insulin-stimulated tyrosine phosphorylation levels of multiple proteins en
dc.status Peer reviewed en

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