Metabolism of retinol in adriamycin-induced cardiomyopathy
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Date
2000-08-01T00:00:00Z
Authors
Danelisen, Igor.
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Abstract
In this study, we have examined the metabolism of vitamin A (retinol) in adriamycin-induced cardiomyopathy in the rat model. Adriamycin was administered in 6 equal i.p. injections over a period of two weeks (cumulative dose 15 mg/kg). Three weeks after the treatment, retinol and 3H-radio labeled retinol were measured in heart, liver, kidney and plasma using HPLC method. In the storage organs, the liver and kidneys, stored amounts of vitamin A were assessed by measuring the levels of retinol palmitate. Activities of retinol mobilizing enzymes, bile salt-dependent retinol ester hydrolase (BSD-REH) and bile salt-independent retinol ester hydrolase (BSI-REH) were also examined in the liver. Levels of _-tocopherol, another known endogenous nonenzymatic antioxidant, were also examined using HPLC method. All of the results were compared with the matching controls. This study shows that retinol levels were strictly maintained in the heart and plasma, even in the severe failure stage. This may be due to the increased mobilization from the storage sites in the liver. Increased consumption in the heart was indicated by an increase in the radio-labeled retinol in this organ as well as in the plasma. The study shows that under condition the radio-labeled retinol in this organ as well as in the plasma. The study shows that under condition of increased myocardial oxidative stress, there is an increased consumption of antioxidant vitamin A. (Abstract shortened by UMI.)