Show simple item record Elhami, Esmat Dietz, Bryson Xiang, Bo Deng, Jixian Wang, Fei Chi, Chao Goertzen, Andrew L Mzengeza, Shadreck Freed, Darren Arora, Rakesh C Tian, Ganghong 2013-11-06T16:07:19Z 2013-11-06T16:07:19Z 2013-10-28
dc.identifier.citation EJNMMI Research. 2013 Oct 28;3(1):72
dc.description.abstract Abstract Background Stem cell therapy has a promising potential for the curing of various degenerative diseases, including congestive heart failure (CHF). In this study, we determined the efficacy of different delivery methods for stem cell administration to the heart for the treatment of CHF. Both positron emission tomography (PET) and magnetic resonance imaging (MRI) were utilized to assess the distribution of delivered stem cells. Methods Adipose-derived stem cells of male rats were labeled with super-paramagnetic iron oxide (SPIO) and 18 F-fluorodeoxyglucose (FDG). The left anterior descending coronary artery (LAD) of the female rats was occluded to induce acute ischemic myocardial injury. Immediately after the LAD occlusion, the double-labeled stem cells were injected into the ischemic myocardium (n = 5), left ventricle (n = 5), or tail vein (n = 4). In another group of animals (n = 3), the stem cells were injected directly into the infarct rim 1 week after the LAD occlusion. Whole-body PET images and MR images were acquired to determine biodistribution of the stem cells. After the imaging, the animals were euthanized and retention of the stem cells in the vital organs was determined by measuring the cDNA specific to the Y chromosome. Results PET images showed that retention of the stem cells in the ischemic myocardium was dependent on the cell delivery method. The tail vein injection resulted in the least cell retention in the heart (1.2% ± 0.6% of total injected cells). Left ventricle injection led to 3.5% ± 0.9% cell retention and direct myocardial injection resulted in the highest rate of cell retention (14% ± 4%) in the heart. In the animals treated 1 week after the LAD occlusion, rate of cell retention in the heart was only 4.5% ±1.1%, suggesting that tissue injury has a negative impact on cell homing. In addition, there was a good agreement between the results obtained through PET-MR imaging and histochemical measurements. Conclusion PET-MR imaging is a reliable technique for noninvasive tracking of implanted stem cells in vivo. Direct injection of stem cells into the myocardium is the most effective way for cell transplantation to the heart in heart failure models.
dc.title Assessment of three techniques for delivering stem cells to the heart using PET and MR imaging
dc.type Journal Article
dc.language.rfc3066 en
dc.description.version Peer Reviewed
dc.rights.holder Esmat Elhami et al.; licensee BioMed Central Ltd. 2013-11-06T16:07:20Z

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