Eugenol attenuates inflammatory response and enhances barrier function during lipopolysaccharide (LPS)-induced inflammation in the porcine intestinal epithelial (IPEC-J2) cells
dc.contributor.author | Hui, Qianru | |
dc.contributor.author | Ammeter, Emily | |
dc.contributor.author | Liu, Shangxi | |
dc.contributor.author | Yang, Runqiang | |
dc.contributor.author | Lu, Peng | |
dc.contributor.author | Lahaya, Ludovic | |
dc.contributor.author | Yang, Chengbo | |
dc.date.accessioned | 2020-08-05T13:52:48Z | |
dc.date.available | 2020-08-05T13:52:48Z | |
dc.date.issued | 2020-08 | |
dc.date.submitted | 2020-08-02T03:22:51Z | en_US |
dc.description.abstract | Eugenol (4-allyl-2-methoxyphenol) is an essential oil component, possessing anti-microbial, anti-inflammatory and anti-oxidative properties, however the effect of eugenol on porcine gut inflammation has not yet been investigated. In this study, an in vitro lipopolysaccharide (LPS)-induced inflammation model in porcine intestinal epithelial cells (IPEC-J2) has been set up. Cells were pre-treated with 100 μM (16.42 mg/L) eugenol for 2 h followed by 10 μg/mL LPS stimulation for 6 h. Pro-inflammatory cytokine secretion, reactive oxygen species, gene expression of pro-inflammatory cytokines, tight junction proteins and nutrient transporters, the expression and distribution of zonula occludens-1 (ZO-1), trans-epithelial electrical resistance (TEER) and cell permeability were measured to investigate the effect of eugenol on inflammatory responses and gut barrier function. The results showed that eugenol pre-treatment significantly suppressed the LPS-stimulated interleukin 8 (IL-8) level and the mRNA abundance of tumor necrosis factor α (TNF-α); restored the LPS-stimulated decrease of mRNA abundance of tight junction proteins zonula occludens-1 (ZO-1), occludin (OCLN) and the mRNA abundance of nutrient transporters B0-system neutral amino acid co-transporter (B0AT1), system ASC sodium-dependent neutral amino acid exchanger 2 (ASCT2), apical sodium-dependent glucose transporter 1 (SGLT1), excitatory amino acid transporter 1 (EAAC1) and peptide transporter 1 (PepT1). In addition, eugenol improved the expression and even redistribution of ZO-1 and tended to increase TEER value and maintained the barrier integrity. In conclusion, a low dose of eugenol attenuated inflammatory responses and enhanced selectively permeable barrier function during LPS-induced inflammation in the IPE-J2 cell line. | en_US |
dc.identifier.uri | http://hdl.handle.net/1993/34842 | |
dc.language.iso | eng | en_US |
dc.rights | open access | en_US |
dc.status | yes | |
dc.subject | Animal Science | en_US |
dc.title | Eugenol attenuates inflammatory response and enhances barrier function during lipopolysaccharide (LPS)-induced inflammation in the porcine intestinal epithelial (IPEC-J2) cells | en_US |
dc.type | Article | en_US |
local.author.affiliation | Rady Faculty of Health Sciences | en_US |