Rapid development of optimized recombinant adenoviral vaccines for biosafety level 4 viruses
| dc.contributor.author | Sahib, Mickey M. | |
| dc.contributor.examiningcommittee | Feldmann, Heinz (Medical Microbiology) Babiuk, Shawn (Immunology) | en |
| dc.contributor.supervisor | Kobinger, Gary (Medical Microbiology) | en |
| dc.date.accessioned | 2010-09-10T17:44:18Z | |
| dc.date.available | 2010-09-10T17:44:18Z | |
| dc.date.issued | 2010-09-10T17:44:18Z | |
| dc.degree.discipline | Medical Microbiology | en_US |
| dc.degree.level | Master of Science (M.Sc.) | en_US |
| dc.description.abstract | This thesis describes the production of adenovirus-based vaccines containing codon-optimized genes from Nipah virus and Crimean-Congo Hemorrhagic Fever virus. Genes encoding envelope proteins from Crimean-Congo Hemorrhagic Fever Virus and Nipah Virus were codon-optimized for translation in human cells and constructed using a modified method of non-gapped gene synthesis, while the entire M segment encoding the glycoprotein precursor for Crimean-Congo Hemorrhagic Fever Virus was commercially synthesized. Genes were cloned into recombinant human adenovirus serotype 5 and the resulting viral particles were amplified, titred and analyzed for in vivo efficacy. Results show that a modified method of non-gapped gene synthesis is an effective and efficient method of producing antigen-encoded DNA and at a fraction of the cost and time required for commercial synthesis. Furthermore, adenovirus-based vaccines induce both cellular and humoral immune responses providing for a highly efficacious vaccine during potential disease outbreaks, where time to completion is of utmost importance. This study has shown that recombinant adenoviral vaccines for Crimean-Congo Hemorrhagic Fever virus and Nipah virus can be produced rapidly and efficiently from virtual DNA sequence to optimized recombinant vaccines in just eight months. | en |
| dc.description.note | October 2010 | en |
| dc.format.extent | 1686701 bytes | |
| dc.format.mimetype | application/pdf | |
| dc.identifier.uri | http://hdl.handle.net/1993/4135 | |
| dc.language.iso | eng | en_US |
| dc.rights | open access | en_US |
| dc.subject | Crimean | en |
| dc.subject | Rift Valley | en |
| dc.subject | Nipah | en |
| dc.subject | vaccine | en |
| dc.subject | immunity | en |
| dc.subject | adenovirus | en |
| dc.title | Rapid development of optimized recombinant adenoviral vaccines for biosafety level 4 viruses | en |
| dc.type | master thesis | en_US |