A robust PCR protocol for HIV drug resistance testing on low-level viremia samples

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dc.contributor.authorGupta, Shivani
dc.contributor.authorTaylor, Tracy
dc.contributor.authorPatterson, Aileen
dc.contributor.authorLiang, Binhua
dc.contributor.authorBullard, Jared
dc.contributor.authorSandstrom, Paul
dc.contributor.authorDomselaar, Gary Van
dc.contributor.authorJi, Hezhao
dc.date.accessioned2017-04-04T19:06:01Z
dc.date.available2017-04-04T19:06:01Z
dc.date.issued2017-04-03
dc.description.abstractThe prevalence of drug resistance (DR) mutations in people with HIV-1 infection, particularly those with low-level viremia (LLV), supports the need to improve the sensitivity of amplification methods for HIV DR genotyping in order to optimize antiretroviral regimen and facilitate HIV-1 DR surveillance and relevant research. Here we report on a fully validated PCR-based protocol that achieves consistent amplification of the protease (PR) and reverse transcriptase (RT) regions of HIV-1 pol gene across many HIV-1 subtypes from LLV plasma samples. HIV-spiked plasma samples from the External Quality Assurance Program Oversight Laboratory (EQAPOL), covering various HIV-1 subtypes, as well as clinical specimens were used to optimize and validate the protocol. Our results demonstrate that this protocol has a broad HIV-1 subtype coverage and viral load span with high sensitivity and reproducibility.Moreover, the protocol is robust even when plasma sample volumes are limited, the HIV viral load is unknown, and/or theHIV subtype is undetermined.Thus, the protocol is applicable for the initial amplification of the HIV-1 PR and RT genes required for subsequent genotypic DR assays.en_US
dc.identifier.doi10.1155/2017/4979252
dc.identifier.urihttp://hdl.handle.net/1993/32187
dc.language.isoengen_US
dc.publisherBioMed Research Internationalen_US
dc.rightsopen accessen_US
dc.subjectHIVen_US
dc.subjectlow-level viremiaen_US
dc.subjectPCRen_US
dc.subjectDrug resistanceen_US
dc.titleA robust PCR protocol for HIV drug resistance testing on low-level viremia samplesen_US
dc.typeArticleen_US
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