Methane emissions and rumen microbial changes in steers fed condensed tannin containing diets under western Canadian conditions

dc.contributor.authorBouchard, Kristen
dc.contributor.examiningcommitteeWittenberg, Karin (Animal Science) Holley, Rick (Food Science) McAllister, Tim (Agriculture and Agri-Food Canada)en_US
dc.contributor.supervisorOminski, Kim (Animal Science) Krause, Denis (Animal Science)en_US
dc.date.accessioned2011-08-26T16:39:47Z
dc.date.available2011-08-26T16:39:47Z
dc.date.issued2011-08-26
dc.degree.disciplineAnimal Scienceen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractA study was conducted to determine if sainfoin, a condensed tannin (CT) containing legume, fed to beef cattle as hay or silage during a western Canadian winter would result in a reduction in methane (CH4) emissions without compromising animal performance. Forty yearling beef steers were fed four diets in a factorial design consisting of two legume forage species (sainfoin or alfalfa) and two preservation methods (silage or hay) over 15 weeks (wks). For each sample wk, animal weight, 24-h CH4 expiration and rumen fluid samples were obtained. Rumen methanogens were characterized using terminal restriction fragment length polymorphisms analysis. Specific bacteria were quantified with real-time polymerase chain reaction analysis. Sainfoin silage (SS) and sainfoin hay (SH) contained 11.9 and 10.5 mg g-1 of CT respectively and supported an acceptable growth rate for backgrounding steers. A decline (P < 0.05) in enteric CH4 formation could only be detected from SH-fed animals compared to alfalfa hay (AH) fed animals when CH4 was expressed as L d-1 or L kg BW-1. The rumen archaeal community structure of experimental animals remained stable regardless of diet type or sample wk. Structural carbohydrate-fermenting bacteria were suppressed in silage diets. Methanogens were less abundant in the rumen fluid samples of steers fed SS but not SH.en_US
dc.description.noteOctober 2011en_US
dc.identifier.urihttp://hdl.handle.net/1993/4779
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectmethaneen_US
dc.subjectcondensed tanninen_US
dc.subjectsainfoinen_US
dc.subjectbeef cattleen_US
dc.titleMethane emissions and rumen microbial changes in steers fed condensed tannin containing diets under western Canadian conditionsen_US
dc.typemaster thesisen_US
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