Plasma Protein Interference at the Dentin-Adhesive Interface

Simple item page
dc.contributor.authorBath, Dr. Karmvir
dc.contributor.authorFranca, Dr. Rodrigo
dc.contributor.authorFiuza, Dr. Cristina
dc.date.accessioned2020-06-12T18:54:39Z
dc.date.available2020-06-12T18:54:39Z
dc.date.issued2020-05-06
dc.date.submitted2020-06-12T18:54:38Zen_US
dc.description.abstractObjective: The purpose of this study is to identify and confirm the presence of macromolecules and their mechanism that interferes with the dentin-adhesive interface. Despite, several advancements of adhesive systems, the bonded interface remains the weakest area of tooth-coloured restorations”. Our goal is to explore the use of chemical analysis via Fourier Transformed Infra-Red (FTIR) to identify bonds formed between adhesive and dentinal fluid protein. Materials & Methods: The protein solutions prepared in this experiment were the Albumin solution (45mg/mL dilution) and IgG solution (25mg/mL dilution) via 0.01M phosphate buffer solution creating constant concentrations as per human plasma. The adhesive systems used in this experiment were Elekta (BisGMA), All- Bond (MDP), Clear-Fill (MDP+MPDP). The experimentation concentrated on the three adhesive types (cured before contact) and then placed in time conditioned contact with protein solution for interactions of instant, 1min, 2min, and 5min. Chemical analysis was completed via FTIR Analysis Infrared spectra (FTIR) using a Thermo Scientific Nicolet™ 6700; in order to understand and identify the bonds formed between adhesive and dentinal fluid protein. Results: Our results show that albumin was detected after 2 minutes in all SEA samples, and IgG after 1 min for ClearFill, 2min for Elekta, and 5 min for Allbond. The detection identifies that a protein coating formation exists on the adhesive surfaces over time, which validates our hypothesis. Conclusion: Serum proteins react with the adhesive surface dependently on the adhesive composition.en_US
dc.description.sponsorshipUniversity of Manitoba Dr. Gerald Niznick College of Dentistry Dental Biomaterials Research Laboratoryen_US
dc.identifier.urihttp://hdl.handle.net/1993/34712
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectDentin-Adhesive Interfaceen_US
dc.subjectPlasma Protein Interferenceen_US
dc.subjectPlasma Protein FTIRen_US
dc.subjectDentin Adhesive Bondingen_US
dc.subjectAdhesive FTIRen_US
dc.titlePlasma Protein Interference at the Dentin-Adhesive Interfaceen_US
dc.typebachelor thesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Bath_Karmvir.pdf
Size:
10.74 MB
Format:
Adobe Portable Document Format
Description:
Thesis Research Paper
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed to upon submission
Description:
Download
Collections
College of Dentistry - B.Sc. (Dent) Projects