Plasma Protein Interference at the Dentin-Adhesive Interface
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Date
2020-05-06
Authors
Bath, Dr. Karmvir
Franca, Dr. Rodrigo
Fiuza, Dr. Cristina
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Abstract
Objective: The purpose of this study is to identify and confirm the presence of
macromolecules and their mechanism that interferes with the dentin-adhesive
interface. Despite, several advancements of adhesive systems, the bonded interface
remains the weakest area of tooth-coloured restorations”. Our goal is to explore the
use of chemical analysis via Fourier Transformed Infra-Red (FTIR) to identify bonds
formed between adhesive and dentinal fluid protein.
Materials & Methods: The protein solutions prepared in this experiment were the
Albumin solution (45mg/mL dilution) and IgG solution (25mg/mL dilution) via
0.01M phosphate buffer solution creating constant concentrations as per human
plasma. The adhesive systems used in this experiment were Elekta (BisGMA), All-
Bond (MDP), Clear-Fill (MDP+MPDP). The experimentation concentrated on the
three adhesive types (cured before contact) and then placed in time conditioned
contact with protein solution for interactions of instant, 1min, 2min, and 5min.
Chemical analysis was completed via FTIR Analysis Infrared spectra (FTIR) using a
Thermo Scientific Nicolet™ 6700; in order to understand and identify the bonds
formed between adhesive and dentinal fluid protein.
Results: Our results show that albumin was detected after 2 minutes in all SEA
samples, and IgG after 1 min for ClearFill, 2min for Elekta, and 5 min for Allbond. The
detection identifies that a protein coating formation exists on the adhesive surfaces
over time, which validates our hypothesis.
Conclusion: Serum proteins react with the adhesive surface dependently on the
adhesive composition.
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Keywords
Dentin-Adhesive Interface, Plasma Protein Interference, Plasma Protein FTIR, Dentin Adhesive Bonding, Adhesive FTIR