dc.contributor.author	Soto-Cerda, Braulio J
dc.contributor.author	Diederichsen, Axel
dc.contributor.author	Ragupathy, Raja
dc.contributor.author	Cloutier, Sylvie
dc.date.accessioned	2013-05-17T03:06:02Z
dc.date.available	2013-05-17T03:06:02Z
dc.date.issued	2013-05-06
dc.date.updated	2013-05-17T03:06:04Z
dc.description.abstract	Abstract Background Flax is valued for its fiber, seed oil and nutraceuticals. Recently, the fiber industry has invested in the development of products made from linseed stems, making it a dual purpose crop. Simultaneous targeting of genomic regions controlling stem fiber and seed quality traits could enable the development of dual purpose cultivars. However, the genetic diversity, population structure and linkage disequilibrium (LD) patterns necessary for association mapping (AM) have not yet been assessed in flax because genomic resources have only recently been developed. We characterized 407 globally distributed flax accessions using 448 microsatellite markers. The data was analyzed to assess the suitability of this core collection for AM. Genomic scans to identify candidate genes selected during the divergent breeding process of fiber flax and linseed were conducted using the whole genome shotgun sequence of flax. Results Combined genetic structure analysis assigned all accessions to two major groups with six sub-groups. Population differentiation was weak between the major groups (FST = 0.094) and for most of the pairwise comparisons among sub-groups. The molecular coancestry analysis indicated weak relatedness (mean = 0.287) for most individual pairs. Abundant genetic diversity was observed in the total panel (5.32 alleles per locus), and some sub-groups showed a high proportion of private alleles. The average genome-wide LD (r 2) was 0.036, with a relatively fast decay of 1.5 cM. Genomic scans between fiber flax and linseed identified candidate genes involved in cell-wall biogenesis/modification, xylem identity and fatty acid biosynthesis congruent with genes previously identified in flax and other plant species. Conclusions Based on the abundant genetic diversity, weak population structure and relatedness and relatively fast LD decay, we concluded that this core collection is suitable for AM studies targeting multiple agronomic and quality traits aiming at the improvement of flax as a true dual purpose crop. Our genomic scans provide the first insights into candidate regions affected by divergent selection in flax. In combination with AM, genomic scans have the ability to increase the power to detect loci influencing complex traits.
dc.description.version	Peer Reviewed
dc.identifier.citation	BMC Plant Biology. 2013 May 06;13(1):78
dc.identifier.doi	http://dx.doi.org/10.1186/1471-2229-13-78
dc.identifier.uri	http://hdl.handle.net/1993/20580
dc.language.rfc3066	en
dc.rights	open access	en_US
dc.rights.holder	Braulio J Soto-Cerda et al.; licensee BioMed Central Ltd.
dc.title	Genetic characterization of a core collection of flax (Linum usitatissimum L.) suitable for association mapping studies and evidence of divergent selection between fiber and linseed types
dc.type	Journal Article

Genetic characterization of a core collection of flax (Linum usitatissimum L.) suitable for association mapping studies and evidence of divergent selection between fiber and linseed types

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