Pretreatment and enzyme hydrolysis of canola meal (Brassica napus L.) and oriental mustard bran (Brassica juncea): production of functional oligosaccharides and impact on phenolic content

Simple item page
dc.contributor.authorYuan, Lin
dc.contributor.examiningcommitteeEskin, Michael (Human Nutritional Sciences) Sapirstein, Harry (Food Science)en_US
dc.contributor.supervisorThiyam-Hollander, Usha (Human Nutritional Sciences) Jones, Peter (Human Nutritional Sciences)en_US
dc.date.accessioned2014-04-19T19:05:29Z
dc.date.available2014-04-19T19:05:29Z
dc.date.issued2014-04-19
dc.degree.disciplineHuman Nutritional Sciencesen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractCanola meal (Brassica napus L.) and oriental mustard bran (Brassica juncea) were subjected to alkali and acid pretreatment to expose pentosan, for enhancing further enzymatic hydrolysis by endo-1,4-β-xylanase from Trichoderma longibrachiatum for the production of oligosaccharides. Pretreatment especially with alkali, effectively increased the relative content of pentosan to about ~ 41% and ~ 72%. Alkali pretreated canola meal and mustard bran resulted in a pentose content of 2.28 ± 0.15 g and 3.20 ± 0.11 g per 100 g substrates at 18 h and 24 h of reaction respectively, which corresponded to ~ 26% and ~ 28% conversion of original pentosan in substrates. UPLC-MS data showed xyloglucuronic acid (XGlcA) as the major oligosaccharide in the hydrolyzates. Reversed-phase HPLC-DAD indicated the principal phenolic compound in the hydrolyzates was sinapine. DPPH radical scavenging assay showed that endoxylananse hydrolyzates of acid pretreated substrates had strong antioxidant activities in comparison to alkali pretreated samples.en_US
dc.description.noteMay 2014en_US
dc.identifier.urihttp://hdl.handle.net/1993/23536
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectoligosaccharidesen_US
dc.subjectphenolicsen_US
dc.subjectenzyme hydrolysisen_US
dc.subjectpretreatmenten_US
dc.subjectcanola mealen_US
dc.subjectmustard branen_US
dc.titlePretreatment and enzyme hydrolysis of canola meal (Brassica napus L.) and oriental mustard bran (Brassica juncea): production of functional oligosaccharides and impact on phenolic contenten_US
dc.typemaster thesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
yuan_lin.pdf
Size:
1.66 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
2.25 KB
Format:
Item-specific license agreed to upon submission
Description:
Download
Collections
FGS - Electronic Theses and Practica