The effects of marginal pyridoxine deficiency and high protein intakes on vitamin b6 status and enzymes in intermediary metabolism in rats
| dc.contributor.author | Raposo_Blouw, Sara | |
| dc.contributor.examiningcommittee | O, Karmin (Animal Sciences) Taylor, Carla (Human Nutritional Sciences) | en_US |
| dc.contributor.supervisor | House, James (Human Nutritional Sciences; Animal Sciences) | en_US |
| dc.date.accessioned | 2015-08-26T12:31:49Z | |
| dc.date.available | 2015-08-26T12:31:49Z | |
| dc.date.issued | 2015 | |
| dc.degree.discipline | Human Nutritional Sciences | en_US |
| dc.degree.level | Master of Science (M.Sc.) | en_US |
| dc.description.abstract | Pyridoxal-5-phosphate (PLP), the active form of vitamin B6 (B6), is a co-factor for enzymes in macronutrient metabolism. Increasing protein intake may affect B6 by increasing PLP-dependent enzymes in amino acid metabolism, which may be more pronounced during moderate B6 deficiency. Decreased B6 status decreases PLP-dependent enzyme activity possibly altering macronutrient metabolism. We examined changing dietary carbohydrate: protein ratios in rats consuming recommended vs. moderately deficient intakes of pyridoxine (PN)-HCl, on plasma markers of B6 status and enzymes in intermediary metabolism. Marginal B6 deficiency decreased all plasma B6 vitamers except for pyridoxic acid. Protein intake (40% energy) significantly reduced plasma PN and tended to decrease plasma pyridoxal with no significant alterations in plasma homocysteine or cysteine. Hepatic cystathionine-γ-lyase, glycogen phosphorylase, plasma aspartate and alanine aminotransferase significantly decreased with marginal B6 deficiency and cystathionine-γ-lyase decreased with increasing protein intake. Marginal B6 deficiency significantly increased hepatic glycogen with no changes in plasma haptoglobin. | en_US |
| dc.description.note | October 2015 | en_US |
| dc.identifier.uri | http://hdl.handle.net/1993/30684 | |
| dc.language.iso | eng | en_US |
| dc.rights | open access | en_US |
| dc.subject | High protein, Vitamin | en_US |
| dc.title | The effects of marginal pyridoxine deficiency and high protein intakes on vitamin b6 status and enzymes in intermediary metabolism in rats | en_US |
| dc.type | master thesis | en_US |