For centuries, Spanish midwives provided essential care to the women in their communities. They were counsellors, wet nurses, and herbalists, as well as, aiding during labor and delivery. However, due to the professionalisation of medicine and the creation of new regulatory bodies, their position within the medical field changed. While midwives were often not allowed to be part of these regulatory bodies, such as the Protomedicato, university-educated physicians used these institutions to assert their dominance over the medical field. Thus, through various marginalisation practices against midwives, university-educated physicians attempted to undermine and limit their role within the newly established medical order. This thesis will explore the different marginalisation practices that these new institutions imposed on midwives, their effects on the trade of midwifery, and how the status of midwives and physicians evolved due to these changes.