Differential innate immunity responses to West Nile virus and bacterial infections in mosquitoes

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dc.contributor.authorMahood, Thomas
dc.contributor.examiningcommitteeDiehl-Jones, William (Biological Sciences) Brassinga, Ann Karen (Microbiology)en_US
dc.contributor.supervisorWhyard, Steve (Biological Sciences)en_US
dc.date.accessioned2013-02-13T15:17:55Z
dc.date.available2013-02-13T15:17:55Z
dc.date.issued2013-02-13
dc.degree.disciplineBiological Sciencesen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractIdentifying the molecular interactions of pathogens in different mosquito species is critical for understanding how mosquitoes transmit diseases. In this study, the role of the Jak-STAT immune signalling pathway in two different mosquito species, (Aedes aegypti L.) and (Culex quinquefasciatus L.) was assessed. Using in silico analysis tools, cell culture, and molecular techniques, changes in gene expression were assessed during lipopolysaccharide (LPS) challenge and West Nile virus (WNV) infection in the two species. It was found that activation of the Jak-STAT pathway occurred more quickly in Ae. aegypti cells compared to Cx. quinquefasciatus cells during LPS exposure. During WNV infections, no significant differences were observed, although preliminary evidence suggests that differential activation of the Jak-STAT pathway may exist between the two species. This research extends our understanding of the mosquito immune system while demonstrating the critical importance of vector-virus interactions across different mosquito species.en_US
dc.description.noteMay 2013en_US
dc.identifier.urihttp://hdl.handle.net/1993/16594
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectMolecular Biologyen_US
dc.subjectJak-STATen_US
dc.subjectAedes aegyptien_US
dc.subjectCulex quinquefasciatusen_US
dc.subjectLPSen_US
dc.subjectBioinformaticsen_US
dc.titleDifferential innate immunity responses to West Nile virus and bacterial infections in mosquitoesen_US
dc.typemaster thesisen_US
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