Multiple environmental factors regulate the expression of the carbohydrate-selective OprB porin of Pseudomonas aeruginosa

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dc.contributor.authorAdewoye, LO
dc.contributor.authorWorobec, EA
dc.date.accessioned2007-10-10T18:11:18Z
dc.date.available2007-10-10T18:11:18Z
dc.date.issued1999-12-31
dc.description.abstractIn response to low extracellular glucose concentration, Pseudomonas aeruginosa induces the expression of the outer membrane carbohydrate-selective OprB porin. The promoter region of the oprB gene was cloned into a lacZ transcriptional fusion vector, and the construct was mobilized into P. aeruginosa OprB-deficient strain, WW100, to evaluate additional environmental factors that influence OprB porin gene expression. Growth temperature, pH of the growth medium, salicylate concentration, and carbohydrate source were found to differentially influence porin expression. This expression pattern was compared to those of whole-cell [C-14]glucose uptake under conditions of high osmolarity, ionicity, variable pH, growth temperatures, and carbohydrate source. These studies revealed that the high-affinity glucose transport genes are down-regulated by salicylic acid, differentially regulated by pH and temperature, and are specifically responsive to exogenous glucose induction.en
dc.format.extent1024656 bytes
dc.format.mimetypeapplication/pdf
dc.identifier.citation0008-4166; CAN J MICROBIOL, DEC 1999, vol. 45, no. 12, p.1033 to 1042.en
dc.identifier.doihttp://dx.doi.org/10.1139/w99-110
dc.identifier.urihttp://hdl.handle.net/1993/2928
dc.language.isoengen_US
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dc.rightsopen accessen_US
dc.statusPeer revieweden
dc.subjectPseudomonas aeruginosaen
dc.subjectOprB porinen
dc.subjectglucose transporten
dc.subjectregulationen
dc.subjectESCHERICHIA-COLI K-12en
dc.subjectOUTER-MEMBRANE PROTEINen
dc.subjectGENE-EXPRESSIONen
dc.subjectTRANSPORT-SYSTEMen
dc.subjectCHANNEL SIZEen
dc.subjectSIGNAL TRANSDUCTIONen
dc.subjectOSMOTIC REGULATIONen
dc.subjectMOLECULAR ANALYSISen
dc.subjectCYSTIC-FIBROSISen
dc.subjectPHen
dc.titleMultiple environmental factors regulate the expression of the carbohydrate-selective OprB porin of Pseudomonas aeruginosaen
dc.typejournal articleen_US
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