Selection of Escherichia coli K88+ specific probiotic strains of E. coli from environmental isolates for post-weaning piglets.
| dc.contributor.author | Setia, Amit | |
| dc.contributor.examiningcommittee | Nyachoti, Charles M (Animal Science) Holley, Rick A (Food Science) | en |
| dc.contributor.supervisor | Krause, Denis O(Animal Science) House, James D (Animal Science) | en |
| dc.date.accessioned | 2007-06-12T14:55:50Z | |
| dc.date.available | 2007-06-12T14:55:50Z | |
| dc.date.issued | 2007-06-12T14:55:50Z | |
| dc.degree.discipline | Animal Science | en_US |
| dc.degree.level | Master of Science (M.Sc.) | en_US |
| dc.description.abstract | Aim of this study was to select environmental E. coli isolates that produced colicins against the swine pathogen E. coli K88+. In initial evaluation using a modified plate method with 18 colicinogenic E. coli constructs, colicins E3, E4, E5, E9, Ia, K and N were found to possess inhibitory activity against 12 ETEC K88+ strains. A total of 463 environmental isolates from cattle rumen, cattle feces, pig feces and hog manure-amended soil were screened for colicin production by a modified plate test. Further, colicinogenic isolates were screened for five toxin genes LT, STa, STb, VT1 and VT2 as well as K88 (F4) fimbriae using PCR reactions. Fourteen non-pathogenic isolates were subjected to characterization of colicin genes by PCR using 9 new primer sequences, antibiotic susceptibilities and substrate utilization. Two potential probiotic strains of E. coli, UM-2 and UM-7 which produced colicins that could utilize potato starch and inulin were selected for in-vitro competition with E. coli K88+ strain 2-12. In vitro competition between the synbiotics and E. coli K88+ revealed inhibition of E. coli K88+. Based on the present in vitro studies it could be concluded that carefully selected potential synbiotics should be further studied for their role in protecting piglets from post-weaning diarrhea without antibiotics. | en |
| dc.description.note | October 2007 | en |
| dc.format.extent | 5837793 bytes | |
| dc.format.mimetype | application/pdf | |
| dc.identifier.uri | http://hdl.handle.net/1993/2456 | |
| dc.language.iso | eng | en_US |
| dc.rights | open access | en_US |
| dc.subject | E. coli K88+ | en |
| dc.subject | Probiotic E. coli | en |
| dc.subject | Colicinogenic | en |
| dc.subject | Environmental E.coli | en |
| dc.title | Selection of Escherichia coli K88+ specific probiotic strains of E. coli from environmental isolates for post-weaning piglets. | en |
| dc.type | master thesis | en_US |