Insulin modulates the electrical activity of dissociated and cultured Subfornical Organ (SFO) neurons in male Sprague Dawley Rats

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dc.contributor.authorLakhi, Suman
dc.contributor.examiningcommitteeHare, James F. (Biological Sciences) Duhamel, Todd (Faculty of Kinesiology and Recreation Management)en_US
dc.contributor.supervisorFry, William M. (Biological Sciences)en_US
dc.date.accessioned2012-01-06T22:56:33Z
dc.date.available2012-01-06T22:56:33Z
dc.date.issued2012-01-06
dc.degree.disciplineBiological Sciencesen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractThe brain is protected by the blood brain barrier (BBB); areas lacking the BBB are termed circumventricular organs (CVOs). The SFO, a CVO is capable of detecting and responding to satiety signals that regulate energy balance. Insulin, a satiety signal, plays a role in energy balance and its actions at the SFO are unknown. The goal was to determine if cultured SFO neurons are electrophysiologically sensitive to insulin. Of 27 neurons tested 33% neurons hyperpolarized (-8.7 ± 1.7 mV), 37% neurons depolarized (10.5 ±2.8 mV) and 30% neurons (8 out of 27) showed no change in membrane potential. Input resistance changes indicated the modulation of two ion channels. Pharmacological data suggests hyperpolarization arises from the opening of KATP channels and depolarization results from the opening of non-selective cationic channels. Thus insulin modulates the electrical activity of SFO neurons and supports that the SFO is a sensor for maintaining energy homeostasis.en_US
dc.description.noteFebruary 2012en_US
dc.identifier.urihttp://hdl.handle.net/1993/5037
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectneuroscienceen_US
dc.subjectelectrophysiologyen_US
dc.subjectinsulinen_US
dc.subjectsubfornical organen_US
dc.subjectobesityen_US
dc.subjectenergy homeostasisen_US
dc.titleInsulin modulates the electrical activity of dissociated and cultured Subfornical Organ (SFO) neurons in male Sprague Dawley Ratsen_US
dc.typemaster thesisen_US
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