The purpose of the present study was to conduct an in-depth investigation of daughters' perspectives on the father-daughter relationship when working together in a family-run business. Because this particular topic is under-studied, it was important to take a qualitative approach to investigate daughters' perspectives on their reasons for joining the family firm, the nature of the work and family conflict with their fathers, their coping strategies for addressing areas of concern, as well as other factors of importance to them. These issues were investigated within the theoretical frameworks of work and family balance theory, and work and family overload theory. Women's perspectives on factors that both assisted and impeded them with successfully balancing a viable career in a family run business with family life were explored. Results from the present study filled an existing gap in the literature, and may also be useful for knowledge translation purposes.