Detection of Antibiotic Resistance Genes in Source and Drinking Water Samples from a First Nations Community in Canada

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dc.contributor.authorFernando, Dinesh M.
dc.contributor.authorTun, Hein Min
dc.contributor.authorPoole, Jenna
dc.contributor.authorPatidar, Rakesh
dc.contributor.authorLi, Ru
dc.contributor.authorMi, Ruidong
dc.contributor.authorAmarawansha, Geethani E. A.
dc.contributor.authorFernando, W. G. Dilantha
dc.contributor.authorKhafipour, Ehsan
dc.contributor.authorFarenhorst, Annemieke
dc.contributor.authorKumara, Ayush
dc.date.accessioned2016-10-04T15:09:12Z
dc.date.available2016-10-04T15:09:12Z
dc.date.issued2016-08
dc.description.abstractAccess to safe drinking water is now recognized as a human right by the United Nations. In developed countries like Canada, access to clean water is generally not a matter of concern. However, one in every five First Nations reserves is under a drinking water advisory, often due to unacceptable microbiological quality. In this study, we analyzed source and potable water from a First Nations community for the presence of coliform bacteria as well as various antibiotic resistance genes. Samples, including those from drinking water sources, were found to be positive for various antibiotic resistance genes, namely, ampC, tet(A), mecA, -lactamase genes (SHV-type, TEM-type, CTX-M-type, OXA-1, and CMY-2-type), and carbapenemase genes (KPC, IMP, VIM, NDM, GES, and OXA-48 genes). Not surprisingly, substantial numbers of total coliforms, including Escherichia coli, were recovered from these samples, and this result was also confirmed using Illumina sequencing of the 16S rRNA gene. These findings deserve further attention, as the presence of coliforms and antibiotic resistance genes potentially puts the health of the community members at risk.en_US
dc.description.sponsorshipThis work was supported by Discovery (A.K., W.G.D.F.) and CreateH2O (A.F.) grants funded by the Natural Science and Engineering Research Council of Canada as well as a University of Manitoba Start-up grant (E.K.). D.M.F. was funded by a Canada Research Chair grant to Peter Loewen, Department of Microbiology, University of Manitoba. R.P. is funded by a postdoctoral fellowship from the Government of Madhya Pradesh, India.en_US
dc.identifier.citationFernando, D.M., Tun, H.M., Poole, J., Patidar, R., LI, R., MI, R., Amarawansha, G.E.A., Fernando, W.G.D., Khafipour, E., Farenhorst, A., Kumar, A. (2016). Detection of antibiotic resistance genes in source and drinking water samples from a First Nation Community in Canada. Applied and Environmental Microbiology. 82(15): 4767-4775.en_US
dc.identifier.urihttp://hdl.handle.net/1993/31870
dc.language.isoengen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.rightsopen accessen_US
dc.subjectdrinking wateren_US
dc.subjecthuman righten_US
dc.subjectantibiotic resistanceen_US
dc.subjectcoliformsen_US
dc.subjectIllumina sequencingen_US
dc.subjectFirst Nations reservesen_US
dc.subjectdrinking water advisoryen_US
dc.subjectmicrobiological qualityen_US
dc.titleDetection of Antibiotic Resistance Genes in Source and Drinking Water Samples from a First Nations Community in Canadaen_US
dc.typeArticleen_US
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