Analysis of restriction fragment length polymorphisms in the ribosomal DNA of a selection of anaerobic chytrids

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dc.contributor.authorHausner, G
dc.contributor.authorInglis, G
dc.contributor.authorYanke, LJ
dc.contributor.authorKawchuk, LM
dc.contributor.authorMcAllister, TA
dc.date.accessioned2007-09-07T18:16:36Z
dc.date.available2007-09-07T18:16:36Z
dc.date.issued2000-07-31T18:16:36Z
dc.description.abstractAnaerobic chytrid strains (representing four genera and seven species based on morphological characters), obtained from a variety of hosts, were analysed for variability within the 5' terminal segment of the ribosomal large subunit (LSU) gene, first internal transcribed spacer (ITS1), and the intergenic spacers (IGS) of the ribosomal repeat unit. In the analyses of the ribosomal LSU gene, strains of Neocallimastix, Orpinomyces, and Anaeromyces clustered into distinct groups, but Piromyces strains did not. Although the size of polymerase chain reaction products for the ITS1 region varied among strains, restriction fragment length polymorphism analysis was not useful in distinguishing between the strains. In the analyses of restriction fragment profiles of the IGS, strains of Orpinomyces and Anaeromyces clustered into distinct groupings that corresponded with morphological characters. In contrast, Neocallimastix strains clustered into two distinct groups. Considerable variability was observed in the IGS for strains of Piromyces. Analysis of this region split strains of Piromyces rhinzinflata obtained from a single bison into at least two distinct genetic groupings. Furthermore, the Piromyces strains obtained from bovine, wapiti, and moose clustered according to host. Overall, our molecular data suggest that morphological characters as observed under the light microscope are insufficient for the designation of phylogenetic species of Neocallimastix and Piromyces, and that integration of molecular and morphological data will be required to establish a natural classification system for the anaerobic members of the Chytridiomycota.en
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dc.format.mimetypeapplication/pdf
dc.identifier.citation0008-4026; CAN J BOT, JUL 2000, vol. 78, no. 7, p.917 to 927.en
dc.identifier.doihttp://dx.doi.org/10.1139/b00-067
dc.identifier.urihttp://hdl.handle.net/1993/2782
dc.language.isoengen_US
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dc.rightsopen accessen_US
dc.statusPeer revieweden
dc.subjectanaerobic fungien
dc.subjectrumenen
dc.subjectChytridiomycotaen
dc.subjectribosomal DNAen
dc.subjectRFLPen
dc.subjectRUMEN FUNGIen
dc.subjectNOV-SPen
dc.subjectPIROMYCES-MAEen
dc.subjectGUT FUNGIen
dc.subjectNEOCALLIMASTIXen
dc.subjectHETEROGENEITYen
dc.subjectDEGRADATIONen
dc.subjectMORPHOLOGYen
dc.subjectGLOMALESen
dc.subjectBIOLOGYen
dc.titleAnalysis of restriction fragment length polymorphisms in the ribosomal DNA of a selection of anaerobic chytridsen
dc.typejournal articleen_US
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