QTL mapping and NIRS estimation of cyanogenic glucosides in flaxseed

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dc.contributor.authorChin-Fatt, Adam
dc.contributor.examiningcommitteeAyele, Belay (Plant Science) Barthet, Véronique (Canadian Grain Commission)en_US
dc.contributor.supervisorCloutier, Sylvie (Plant Science) Duguid, Scott (Plant Science)en_US
dc.date.accessioned2014-08-22T18:05:04Z
dc.date.available2014-08-22T18:05:04Z
dc.date.issued2014-08-22
dc.degree.disciplinePlant Scienceen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractCyanogenic glucosides (CGs) are bioactive plant secondary metabolites that can release toxic hydrocyanic acid when hydrolyzed. The accumulation of CGs in flax seed is a safety issue as a feed component and may contravene international trade restrictions. Here, we report the identification of major effect quantitative trait loci (QTL) for linustatin and neolinustatin, the two most abundant CGs in the mature flax seed. Current methods of CG analysis for screening and quality control are prohibitively expensive. Here, we also report on the development of a low cost, high throughput method of analysis using near infrared spectroscopy (NIRS) to estimate individual CGs and total hydrocyanic acid equivalent based on a regression of reference data obtained by gas chromatography. The genetic and physical mapping of the QTL and the NIRS calibration hold direct applications in the development of a flax breeding strategy for developing germplasm and cultivars with reduced CG content.en_US
dc.description.noteOctober 2014en_US
dc.identifier.urihttp://hdl.handle.net/1993/23856
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectcyanogenicen_US
dc.subjectflaxen_US
dc.subjectlinustatinen_US
dc.subjectneolinustatinen_US
dc.subjectQTLen_US
dc.subjectspectroscopyen_US
dc.titleQTL mapping and NIRS estimation of cyanogenic glucosides in flaxseeden_US
dc.typemaster thesisen_US
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