Mechanisms of laminar organization of hippocampal excitatory synapses

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dc.contributor.authorAbrar Basha, Mohammed
dc.contributor.examiningcommitteeDhingra, Sanjiv (Physiology and Pathophysiology)
dc.contributor.examiningcommitteeStobart, Jillian (Pharmacy)
dc.contributor.supervisorSiddiqui, Tabrez
dc.date.accessioned2024-04-04T14:47:01Z
dc.date.available2024-04-04T14:47:01Z
dc.date.issued2024-03-28
dc.date.submitted2024-03-28T20:26:27Zen_US
dc.date.submitted2024-04-03T21:23:43Zen_US
dc.degree.disciplinePhysiology and Pathophysiology
dc.degree.levelMaster of Science (M.Sc.)
dc.description.abstractThe hippocampus has well-defined laminae with specified neuronal pathways. In the hippocampus, I investigated the developmental expression and functional roles of prominent synapse organizers, leucine-rich-repeat transmembrane neuronal proteins (LRRTMs). LRRTM1 and LRRTM2 are compartmentalised to the stratum radiatum (SR) and stratum lacunosum moleculare (SLM), respectively, with specific expression impacting synaptic architecture and cognitive behaviours. Using immunohistochemistry, I demonstrated that LRRTM1 and LRRTM2 exhibit distinct temporal and spatial expression patterns in the hippocampal laminae: LRRTM1 is predominantly localized in the SR, and LRRTM2 in the SLM. My findings indicate that LRRTM2 expression in the SLM corresponds with excitatory synapse formation and maturation in the cortical temporoammonic cortical inputs to the CA1. However, the functional significance of LRRTM2 compartmentalization in the SLM was not known. To this end, I conducted a suite of behavioural assays in mice lacking LRRTM2 in the dorsal CA1 (Lrrtm2-CA1-cKO). My studies revealed that Lrrtm2-CA1-cKO mice display anxiety-associated phenotype, but only in female mice.
dc.description.noteMay 2024
dc.identifier.urihttp://hdl.handle.net/1993/38147
dc.language.isoeng
dc.rightsopen accessen_US
dc.subjectNeuroscience
dc.titleMechanisms of laminar organization of hippocampal excitatory synapses
dc.typemaster thesisen_US
local.subject.manitobano
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