Characterization of anti-ricin monoclonal antibodies and the construction of a chimeric murine-human IgG2/K anti-ricin monoclonal antibody
| dc.contributor.author | Vendramelli, Robert Matthew | |
| dc.contributor.examiningcommittee | Corbett, Cindi (Medical Microbiology) Yao, Xiaojian (Medical Microbiology) Soussi-Gounni, Abdelilah (Immunology) | en |
| dc.contributor.supervisor | Berry, Jody (Medical microbiology) | en |
| dc.date.accessioned | 2011-04-12T17:57:07Z | |
| dc.date.available | 2011-04-12T17:57:07Z | |
| dc.date.issued | 2011-04-12T17:57:07Z | |
| dc.degree.discipline | Medical Microbiology | en_US |
| dc.degree.level | Master of Science (M.Sc.) | en_US |
| dc.description.abstract | Ricin toxin is a very deadly plant protein that is synthesized by the plant Ricinus communis. The molecular structure of ricin toxin places it in a group of similar proteins classified as a Type II RIP due to its heterodimeric construction; it is composed of a toxic A-chain possessing enzymatic action, and a receptor binding B-chain. Monoclonal antibodies were obtained with binding activities against either the A-chain or B-chain, and a surrogate non-toxic ricin analogue, TST10114, was determined to be suitable for characterization of the anti-ricin monoclonal antibodies. One potent anti-ricin A-chain neutralizing monoclonal antibody was chosen for chimerization, RAC18, which exhibited strong binding affinity and neutralizing properties. The constant regions of a human immunoglobulin G2 (IgG2) were used as the backbone for the recombinant chimeric antibody. The resulting chimeric RAC18-huG2 was transiently expressed in human-derived HEK 293F cells, purified, and assessed for binding characteristics and functional attributes. | en |
| dc.description.note | May 2011 | en |
| dc.format.extent | 1969298 bytes | |
| dc.format.mimetype | application/pdf | |
| dc.identifier.uri | http://hdl.handle.net/1993/4526 | |
| dc.language.iso | eng | en_US |
| dc.rights | open access | en_US |
| dc.subject | Immunology | en |
| dc.subject | ricin | en |
| dc.subject | toxin | en |
| dc.subject | monoclonal | en |
| dc.subject | antibody | en |
| dc.subject | chimeric | en |
| dc.title | Characterization of anti-ricin monoclonal antibodies and the construction of a chimeric murine-human IgG2/K anti-ricin monoclonal antibody | en |
| dc.type | master thesis | en_US |