NERD-seq: a novel approach of Nanopore direct RNA sequencing that expands representation of non-coding RNAs
| dc.contributor.author | Saville, Luke | |
| dc.contributor.author | Wu, Li | |
| dc.contributor.author | Habtewold, Jemaneh | |
| dc.contributor.author | Cheng, Yubo | |
| dc.contributor.author | Gollen, Babita | |
| dc.contributor.author | Mitchell, Liam | |
| dc.contributor.author | Stuart-Edwards, Matthew | |
| dc.contributor.author | Haight, Travis | |
| dc.contributor.author | Mohajerani, Majid | |
| dc.contributor.author | Zovoilis, Athanasios | |
| dc.date.accessioned | 2024-09-03T14:39:32Z | |
| dc.date.available | 2024-09-03T14:39:32Z | |
| dc.date.issued | 2024-08-28 | |
| dc.date.updated | 2024-09-01T03:21:34Z | |
| dc.description.abstract | Abstract Non-coding RNAs (ncRNAs) are frequently documented RNA modification substrates. Nanopore Technologies enables the direct sequencing of RNAs and the detection of modified nucleobases. Ordinarily, direct RNA sequencing uses polyadenylation selection, studying primarily mRNA gene expression. Here, we present NERD-seq, which enables detection of multiple non-coding RNAs, excluded by the standard approach, alongside natively polyadenylated transcripts. Using neural tissues as a proof of principle, we show that NERD-seq expands representation of frequently modified non-coding RNAs, such as snoRNAs, snRNAs, scRNAs, srpRNAs, tRNAs, and rRFs. NERD-seq represents an RNA-seq approach to simultaneously study mRNA and ncRNA epitranscriptomes in brain tissues and beyond. | |
| dc.identifier.citation | Genome Biology. 2024 Aug 28;25(1):233 | |
| dc.identifier.uri | https://doi.org/10.1186/s13059-024-03375-8 | |
| dc.identifier.uri | http://hdl.handle.net/1993/38487 | |
| dc.language.rfc3066 | en | |
| dc.rights | open access | en_US |
| dc.rights.holder | The Author(s) | |
| dc.title | NERD-seq: a novel approach of Nanopore direct RNA sequencing that expands representation of non-coding RNAs | |
| dc.type | Journal Article |