TILRR Promotes Migration of Immune Cells Through Induction of Soluble Inflammatory Mediators
dc.contributor.author | Kashem, Mohammad | |
dc.contributor.author | Ren, Xiaoou | |
dc.contributor.author | Li, Hongzhao | |
dc.contributor.author | Liang, Binhua | |
dc.contributor.author | Li, Lin | |
dc.contributor.author | Lin, Francis | |
dc.contributor.author | Plummer, Francis | |
dc.contributor.author | Luo, Ma | |
dc.date.accessioned | 2021-08-12T16:10:17Z | |
dc.date.available | 2021-08-12T16:10:17Z | |
dc.date.issued | 2020-07-03 | |
dc.date.submitted | 2021-08-12T01:32:25Z | en_US |
dc.description.abstract | TILRR has been identified as an important modulator of inflammatory responses. It is associated with NF-kB activation and inflammation. Our previous study showed that TILRR significantly increased the expression of many innate immune responsive genes and increased the production of several pro-inflammatory cytokines/chemokines by cervical epithelial cells. In this study, we evaluated the effect of TILRR-induced proinflammatory cytokines/chemokines on the migration of immune cells. The effect of culture supernatants of TILRR-overexpressed cervical epithelial cells on the migration of THP-1 monocytes and MOLT-4 T-lymphocytes was evaluated using Transwell assay and a novel microfluidic device. We showed that the culture supernatants of TILRR-overexpressed HeLa cells attracted significantly more THP-1 cells (11–40%, p = 0.0004–0.0373) and MOLT-4 cells (14–17%, p = 0.0010–0.0225) than that of controls. The microfluidic device-recorded image analysis showed a significantly higher amount with a longer mean cell migration distance of THP-1 (p < 0.0001–0.0180) and MOLT-4 (p < 0.0001–0.0025) cells was observed toward the supernatants of TILRR-overexpressed cervical epithelial cells compared to that of the controls. Thus, the cytokines/chemokines secreted by the TILRR-overexpressed cervical epithelial cells attracted immune cells, such as monocytes and T cells, and may potentially influence immune cell infiltration in tissues. | en_US |
dc.description.sponsorship | The study was funded by an operating grant from the Canadian Institutes of Health Research (CIHR), operating grant - PA: CHVI Vaccine Discovery and Social Research (http://www.cihrirsc. gc.ca/e/193.html) and by a discovery grant from the Natural Sciences and Engineering Research Council of Canada (NSERC) (RGPIN-2014-04789). | en_US |
dc.identifier.citation | Kashem MA, Ren X, Li H, Liang B, Li L, Lin F, Plummer FA and Luo M (2020) TILRR Promotes Migration of Immune Cells Through Induction of Soluble Inflammatory Mediators. Front. Cell Dev. Biol. 8:563. doi: 10.3389/fcell.2020.00563 | en_US |
dc.identifier.doi | 10.3389/fcell.2020.00563 | |
dc.identifier.uri | http://hdl.handle.net/1993/35795 | |
dc.language.iso | eng | en_US |
dc.publisher | Frontiers | en_US |
dc.rights | open access | en_US |
dc.subject | TILRR | en_US |
dc.subject | pro-inflammatory cytokines/chemokines | en_US |
dc.subject | cervical epithelial cell culture supernatants | en_US |
dc.subject | THP-1 | en_US |
dc.subject | MOLT-4 | en_US |
dc.subject | Transwell assay | en_US |
dc.subject | microfluidic device | en_US |
dc.title | TILRR Promotes Migration of Immune Cells Through Induction of Soluble Inflammatory Mediators | en_US |
dc.type | Article | en_US |
local.author.affiliation | Rady Faculty of Health Sciences | en_US |