TILRR Promotes Migration of Immune Cells Through Induction of Soluble Inflammatory Mediators

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dc.contributor.authorKashem, Mohammad
dc.contributor.authorRen, Xiaoou
dc.contributor.authorLi, Hongzhao
dc.contributor.authorLiang, Binhua
dc.contributor.authorLi, Lin
dc.contributor.authorLin, Francis
dc.contributor.authorPlummer, Francis
dc.contributor.authorLuo, Ma
dc.date.accessioned2021-08-12T16:10:17Z
dc.date.available2021-08-12T16:10:17Z
dc.date.issued2020-07-03
dc.date.submitted2021-08-12T01:32:25Zen_US
dc.description.abstractTILRR has been identified as an important modulator of inflammatory responses. It is associated with NF-kB activation and inflammation. Our previous study showed that TILRR significantly increased the expression of many innate immune responsive genes and increased the production of several pro-inflammatory cytokines/chemokines by cervical epithelial cells. In this study, we evaluated the effect of TILRR-induced proinflammatory cytokines/chemokines on the migration of immune cells. The effect of culture supernatants of TILRR-overexpressed cervical epithelial cells on the migration of THP-1 monocytes and MOLT-4 T-lymphocytes was evaluated using Transwell assay and a novel microfluidic device. We showed that the culture supernatants of TILRR-overexpressed HeLa cells attracted significantly more THP-1 cells (11–40%, p = 0.0004–0.0373) and MOLT-4 cells (14–17%, p = 0.0010–0.0225) than that of controls. The microfluidic device-recorded image analysis showed a significantly higher amount with a longer mean cell migration distance of THP-1 (p < 0.0001–0.0180) and MOLT-4 (p < 0.0001–0.0025) cells was observed toward the supernatants of TILRR-overexpressed cervical epithelial cells compared to that of the controls. Thus, the cytokines/chemokines secreted by the TILRR-overexpressed cervical epithelial cells attracted immune cells, such as monocytes and T cells, and may potentially influence immune cell infiltration in tissues.en_US
dc.description.sponsorshipThe study was funded by an operating grant from the Canadian Institutes of Health Research (CIHR), operating grant - PA: CHVI Vaccine Discovery and Social Research (http://www.cihrirsc. gc.ca/e/193.html) and by a discovery grant from the Natural Sciences and Engineering Research Council of Canada (NSERC) (RGPIN-2014-04789).en_US
dc.identifier.citationKashem MA, Ren X, Li H, Liang B, Li L, Lin F, Plummer FA and Luo M (2020) TILRR Promotes Migration of Immune Cells Through Induction of Soluble Inflammatory Mediators. Front. Cell Dev. Biol. 8:563. doi: 10.3389/fcell.2020.00563en_US
dc.identifier.doi10.3389/fcell.2020.00563
dc.identifier.urihttp://hdl.handle.net/1993/35795
dc.language.isoengen_US
dc.publisherFrontiersen_US
dc.rightsopen accessen_US
dc.subjectTILRRen_US
dc.subjectpro-inflammatory cytokines/chemokinesen_US
dc.subjectcervical epithelial cell culture supernatantsen_US
dc.subjectTHP-1en_US
dc.subjectMOLT-4en_US
dc.subjectTranswell assayen_US
dc.subjectmicrofluidic deviceen_US
dc.titleTILRR Promotes Migration of Immune Cells Through Induction of Soluble Inflammatory Mediatorsen_US
dc.typeArticleen_US
local.author.affiliationRady Faculty of Health Sciencesen_US
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