Sex-based diverse plaque microbiota in children with severe caries

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dc.contributor.authorCruz de Jesus, Vivianne
dc.contributor.authorShikder, Rayhan
dc.contributor.authorOryniak, Derek
dc.contributor.authorMann, Kelsey
dc.contributor.authorAlamri, Abeer
dc.contributor.authorMittermuller, Betty-Anne
dc.contributor.authorDuan, Kangmin
dc.contributor.authorHu, Pingzhao
dc.contributor.authorSchroth, Robert J
dc.contributor.authorChelikani, Prashen
dc.date.accessioned2020-06-17T18:53:41Z
dc.date.available2020-06-17T18:53:41Z
dc.date.issued2020
dc.date.submitted2020-06-10T16:05:59Zen_US
dc.description.abstractSevere early childhood caries (S-ECC) is a multifactorial disease that can lead to suffering and reduced oral health related quality of life in young children. The bacterial and fungal composition of dental plaque and how children`s sex is associated with S-ECC are largely unknown. In this study, V4-16S rRNA and ITS1 rRNA gene amplicon sequencing was used to compare the plaque bacteriome and mycobiome of children <72 months of age; 40 with S-ECC (15 males, 25 females) and 40 caries-free (19 males, 21 females). Health- and nutrition-related questionnaire data were also investigated. This study aimed to analyze potential sex-based differences in the supragingival plaque microbiota of young children with S-ECC and those caries-free. Behavioral and nutritional habit differences were observed between children with SECC and those caries-free and between male and female children. Overall, higher levels of Veillonella dispar, Streptococcus mutans and other bacterial species, were found in the S-ECC group compared to caries-free controls (p < 0.05). A significant difference in the abundance of Neisseria was observed between males and females with S-ECC (p < 0.05). Fungal taxonomic analysis showed significantly higher levels of Candida dubliniensis in the plaque of children with S-ECC compared to caries-free (p < 0.05), but no differences were observed with Ca. albicans (p > 0.05). Significant differences in the relative abundance of Mycosphaerella, Cyberlindnera and Trichosporon fungal species were also observed between the caries-free and S-ECC groups (p < 0.05). Machine learning analysis revealed the most important bacterial and fungal species for classifying S-ECC versus caries-free. Different patterns of crosstalk between microbial species were observed between male and female children. Our work demonstrates that plaque microbiota and sex may be important determinants for S-ECC and could be factors to consider for inclusion in caries risk assessment tools.en_US
dc.description.sponsorshipOperating grants from the Children’s Hospital Research Institute of Manitoba (CHRIM) and the Canadian Institute of Health Research (CIHR)en_US
dc.identifier.urihttp://hdl.handle.net/1993/34717
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectDental caries; child, preschool; human microbiome; fungi; bacteria; machine learningen_US
dc.titleSex-based diverse plaque microbiota in children with severe cariesen_US
dc.typeArticleen_US
local.author.affiliationRady Faculty of Health Sciencesen_US
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