Antibiotic Resistance and Expression Of Resistance-Nodulation-Division Pump- and Outer Membrane Porin-Encoding Genes in Acinetobacter Species Isolated from Canadian Hospitals

Simple item page
dc.contributor.authorFernando, Dinesh
dc.contributor.authorZhanel, George
dc.contributor.authorKumar, Ayush
dc.date.accessioned2016-06-07T19:13:49Z
dc.date.available2016-06-07T19:13:49Z
dc.date.issued2013-1-1
dc.date.updated2016-06-07T06:54:18Z
dc.description.abstractBACKGROUND: Bacterial pathogens belonging to the genus Acinetobacter cause serious infections in immunocompromised individuals that are very difficult to treat due to their extremely high resistance to many antibiotics.OBJECTIVE: To investigate the role of resistance-nodulation-division (RND) pumps and porins in the antibiotic resistance of Acinetobacter species collected from Canadian hospitals.METHODS: Clinical isolates of Acinetobacter species collected from Canadian hospitals were analyzed for the expression of genes encoding RND pumps (adeB, adeG, adeJ, AciBau_2746 and AciBau_2436) and outer membrane porins (carO, 33 kDa porin and oprD) using quantitative reverse transcription (qRT) polymerase chain reaction. Species identification of the isolates was performed using a multiplex polymerase chain reaction method for gyrB.RESULTS: The expression of RND pump-encoding genes was widespread in the clinical isolates of Acinetobacter species, with each of the isolates expressing at least one RND pump. adeG was found to be overexpressed in all of the isolates, while adeB was found to be overexpressed in only two isolates. Among the porin-encoding genes, the expression of carO was considerably downregulated among the majority of isolates.CONCLUSION: The present study was the first to analyze the expression of RND pump- and porin-encoding genes in the clinical isolates of Acinetobacter species from Canadian hospitals. The overexpression of genes encoding RND pumps and the downregulation of genes encoding porins was common in clinical isolates of Acinetobacter species from Canadian hospitals, with the AdeFGH pump being the most commonly expressed RND pump.
dc.description.versionPeer Reviewed
dc.identifier.citationDinesh Fernando, George Zhanel, and Ayush Kumar, “Antibiotic Resistance and Expression Of Resistance-Nodulation-Division Pump- and Outer Membrane Porin-Encoding Genes in Acinetobacter Species Isolated from Canadian Hospitals,” Canadian Journal of Infectious Diseases and Medical Microbiology, vol. 24, no. 1, pp. 17-21, 2013. doi:10.1155/2013/696043
dc.identifier.urihttp://dx.doi.org/10.1155/2013/696043
dc.identifier.urihttp://hdl.handle.net/1993/31350
dc.language.rfc3066en
dc.rightsopen accessen_US
dc.rights.holderCopyright © 2013 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.titleAntibiotic Resistance and Expression Of Resistance-Nodulation-Division Pump- and Outer Membrane Porin-Encoding Genes in Acinetobacter Species Isolated from Canadian Hospitals
dc.typeJournal Article
Files
Original bundle
Now showing 1 - 2 of 2
Thumbnail Image
Name:
CJIDMM.2013.696043.xml
Size:
12.25 KB
Format:
Extensible Markup Language
Description:
Download
Thumbnail Image
Name:
CJIDMM.2013.696043.pdf
Size:
665.09 KB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
2.24 KB
Format:
Item-specific license agreed to upon submission
Description:
Download
Collections
University of Manitoba Scholarship
Rady Faculty of Health Sciences Scholarly Works