Calcium-sensing Receptor is not Expressed in the Absorptive Enterocytes of Weaned Piglets

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dc.contributor.authorZhao, Xiaoya
dc.contributor.authorHui, Qianqu
dc.contributor.authorAzevedo, Paula A
dc.contributor.authorNyachoti, Charles M.
dc.contributor.authorO, Karmin
dc.contributor.authorYang, Chengbo
dc.date.accessioned2022-04-01T14:25:36Z
dc.date.available2022-04-01T14:25:36Z
dc.date.issued2022-03-16
dc.date.submitted2022-03-19T21:34:48Zen_US
dc.description.abstractThe calcium-sensing receptor (CaSR) is a kokumi receptor that plays an essential role in nutrient sensing and animal physiology, growth, and development. Pig CaSR (pCaSR) was identified and characterized in the intestine. However, further research is still needed to confirm the expression of CaSR in the epithelial cells isolated from weaned piglets. In this study, primary enterocytes were isolated and characterized from the ileum of weaned piglets by the Weiser distended intestinal sac technique and fluorescence-activated cell sorting (FACS) based on sucrase-isomaltase as an enterocyte-specific marker. The expression of CaSR was investigated in both primary enterocytes and the intestinal porcine enterocyte cell line (IPEC-J2) by droplet digital PCR (ddPCR), immunofluorescence staining, and western blotting. Results demonstrated that porcine enterocytes could be obtained using FACS with the sucrase-isomaltase as the enterocyte-specific marker and that pCaSR is not expressed in both porcine ileal enterocytes and IPEC-J2 cells, which specifically identified the expression of pCaSR in ileal enterocytes with sensitive and specific approaches.en_US
dc.description.sponsorshipCanada foundation for innovation (CFI), the University of Manitoba Start-Up Grant, the Graduate Enhancement of Tri-Council Stipends (GETS) programen_US
dc.identifier.doi10.1093/jas/skac085
dc.identifier.urihttp://hdl.handle.net/1993/36392
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectCalcium-sensing receptoren_US
dc.subjectenterocytesen_US
dc.subjectenteroendocrine cellsen_US
dc.subjectIPEC-J2en_US
dc.subjectweaned pigletsen_US
dc.titleCalcium-sensing Receptor is not Expressed in the Absorptive Enterocytes of Weaned Pigletsen_US
dc.typepreprinten_US
local.author.affiliationFaculty of Agricultural and Food Sciences::Department of Animal Scienceen_US
oaire.awardNumberRGPIN-2017-04967en_US
oaire.awardTitleNatural Sciences and Engineering Council of Canada (NSERC) Discovery Grantsen_US
oaire.citation.issueskac085en_US
oaire.citation.titleJournal of Animal Scienceen_US
project.funder.identifierhttps://doi.org/10.13039/501100000038en_US
project.funder.nameNatural Sciences and Engineering Council of Canadaen_US
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