Evaluation of in vitro methodology to determine protein digestibility and quality of plant-based proteins

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dc.contributor.authorFranczyk, Adam
dc.contributor.examiningcommitteeMollard, Rebecca (Food and Human Nutritional Sciences)en_US
dc.contributor.examiningcommitteeJoseph, Sijo (Food and Human Nutritional Sciences)en_US
dc.contributor.examiningcommitteeYang, Chengbo (Animal Science)en_US
dc.contributor.supervisorHouse, James (Food and Human Nutritional Sciences)en_US
dc.date.accessioned2018-09-18T15:58:29Z
dc.date.available2018-09-18T15:58:29Z
dc.date.issued2018-08-29en_US
dc.date.submitted2018-09-16T01:43:03Zen
dc.degree.disciplineFood and Human Nutritional Sciencesen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractProtein quality evaluations currently require that an animal bioassay be performed to determine true protein digestibility (TPD). This measure is used to calculate the protein digestibility corrected amino acid score (PDCAAS) prior to qualifying for a protein content claim. Several in vitro assays evaluating protein digestibility that currently reside in literature have not been used to assess protein quality, hence this thesis was undertaken to evaluate the pH-drop (PHD) and two-step digestion (TSD) method to determine PDCAAS in vitro on 65 plant-based proteins composed of different species, variety and heat treatment. Strong significant correlations were indicated by both in vitro protein quality calculations for the PHD (R = 0.91; P ≥ 0.0001) and the TSD (R = 0.92; P ≥ 0.0001) with the PDCAAS. This relationship suggests that either method to evaluate protein digestibility may be reliable to calculate PDCAAS in vitro, thus reducing the time, expense and ethical quandaries that currently apply to evaluating protein quality content claims.en_US
dc.description.noteOctober 2018en_US
dc.identifier.urihttp://hdl.handle.net/1993/33437
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectProtein qualityen_US
dc.subjectPlant proteinen_US
dc.subjectIn vitro protein digestibilityen_US
dc.subjectIn vitro protein qualityen_US
dc.titleEvaluation of in vitro methodology to determine protein digestibility and quality of plant-based proteinsen_US
dc.typemaster thesisen_US
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