Observation of infectious Legionella pneumophila in host model Caenorhabditis elegans

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dc.contributor.authorHellinga, Jacqueline
dc.contributor.examiningcommitteeSparling, Richard (Microbiology) Kormish, Jay (Biological Sciences)en_US
dc.contributor.supervisorBrassinga, Ann Karen C. (Microbiology)en_US
dc.date.accessioned2014-08-20T17:01:46Z
dc.date.available2014-08-20T17:01:46Z
dc.date.issued2014-08-20
dc.degree.disciplineMicrobiologyen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractThe Gram-negative bacterium Legionella pneumophila is an intracellular parasite of aquatic protozoa. It exhibits a distinct dimorphic lifecycle that alternates between vegetative replicative form (RF) and infectious cyst-like form (CLF). Inadvertent inhalation of aerosolized CLFs by immunocompromised individuals leads to an infection in alveolar macrophages causing Legionnaires' disease. To further study a Legionella infection the use of the multicellular organism Caenorhabditis elegans was done. Differential Interference Contrast (DIC) microscopy of live L. pneumophila infected nematodes shows Legionella-containing vacuoles (LCVs) with motile forms. Transmission Electron Microscopy (TEM) defined the ultrastructure of L. pneumophila forms found in the primary infection site of the intestinal lumen and the secondary infection site in the gonadal tissues. These findings suggest the possible intracellular replication cycle of Legionella occurring in the gonadal tissues of the nematode. Providing insight and a plausible evolutionary origin of the ability of L. pneumophila to manipulate the macrophage innate immune system.en_US
dc.description.noteOctober 2014en_US
dc.identifier.urihttp://hdl.handle.net/1993/23835
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectC. elegansen_US
dc.subjectLegionellaen_US
dc.subjectTEMen_US
dc.subjectDICen_US
dc.subjectapoptosisen_US
dc.subjectinnate immuneen_US
dc.subjectendocytosisen_US
dc.subjectinfectionen_US
dc.titleObservation of infectious Legionella pneumophila in host model Caenorhabditis elegansen_US
dc.typemaster thesisen_US
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