Conjugation of tetanus toxoid with Salmonella typhimurium PTCC 1735 O-specific polysaccharide and its effects on production of opsonizing antibodies in a mouse model

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dc.contributor.authorJazani, NH
dc.contributor.authorWorobec, EA
dc.contributor.authorShahabi, S
dc.contributor.authorNejad, GB
dc.date.accessioned2007-10-10T18:13:32Z
dc.date.available2007-10-10T18:13:32Z
dc.date.issued2005-04-30
dc.description.abstractSerious enteric and extra-intestinal infections with Salmonella typhimurium are very common in many human populations. Phagocytosis is the main defense mechanism against this bacterium; however, the unique structure of S. typhimurium lipopolysaccharide (LPS) makes it resistant to opsonization by complement components. In the present study, the S. typhimurium LPS O-chain was purified and conjugated with tetanus toxoid and the effects of the conjugated vaccine (O-specific polysaccharide tetanus toxoid (O-SP-TT)) on induction of specific antibodies were investigated in a mouse model. In vitro assays measuring phagocytosis in the presence of opsonizing antibodies were performed. Three subcutaneous injections of the O-SP-TT conjugate conferred protection against the intraperitoneal challenge with S. typhimurium and the LD50 was greater for immunized animals than for controls. The mean number of ingested S. typhimirium / mouse peritonea] cell in the presence of sera obtained from immunized mice with purified O-chain, O-SP-TT conjugate, heat-killed bacteria, and negative control were 6.96, 14.24, 15.96, and 6.67, respectively. In summary, we developed an O-SP-TT conjugate that induced opsonizing antibodies that increased phagocytosis, as determined by in vitro assays. In addition, chemiluminescence results, an indicator of oxidative burst, indicated that peritoneal cells respond better to live S. typhimurium cells in the presence of sera obtained from O-SP-TT conjugate immunized mice.en
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dc.identifier.citation0008-4166; CAN J MICROBIOL, APR 2005, vol. 51, no. 4, p.319 to 324.en
dc.identifier.doihttp://dx.doi.org/10.1139/w05-008
dc.identifier.urihttp://hdl.handle.net/1993/2929
dc.language.isoengen_US
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dc.rightsopen accessen_US
dc.statusPeer revieweden
dc.subjectlipopolysaccharideen
dc.subjectconjugateen
dc.subjectS. typhimuriumen
dc.subjectvaccineen
dc.subjectLIPOPOLYSACCHARIDESen
dc.titleConjugation of tetanus toxoid with Salmonella typhimurium PTCC 1735 O-specific polysaccharide and its effects on production of opsonizing antibodies in a mouse modelen
dc.typejournal articleen_US
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