Regulation of wheat seed germination and seedling growth by brassinosteroid
dc.contributor.author | Kaur, Rupinder | |
dc.contributor.examiningcommittee | Jordan, Mark (Plant Science) | en_US |
dc.contributor.examiningcommittee | Schroeder, Dana (Biological Science) | en_US |
dc.contributor.supervisor | Ayele, Belay (Plant Science) | en_US |
dc.date.accessioned | 2019-11-15T17:00:59Z | |
dc.date.available | 2019-11-15T17:00:59Z | |
dc.date.issued | 2019-10-24 | en_US |
dc.date.submitted | 2019-11-06T18:09:50Z | en |
dc.degree.discipline | Plant Science | en_US |
dc.degree.level | Master of Science (M.Sc.) | en_US |
dc.description.abstract | Brassinosteroids (BRs) are plant hormones known to play a role in regulation of seed germination and seedling growth, and these roles of BR involve its interaction with other phytohormones such as abscisic acid (ABA) and gibberellin (GA). This study analyzed the effects of inhibition of BR biosynthesis on seed germination and seedling growth, and on expression of genes involved in the metabolism of two other phytohormones: ABA, a germination inhibitor and GA, germination enhancers. Treatment with BR biosynthesis inhibitor has no effect on seed germination, but it reduced seedling growth. Co-application of the BR biosynthesis inhibitor and exogenous BR rescued the coleoptile growth but caused reduction of root growth in later phases of imbibition as compared to the control or treatment with BR biosynthesis inhibitor alone. Treatment with BR biosynthesis inhibitor affected the expression of ABA metabolic NCED2, CYP707A1, CYP707A2 and GA biosynthetic GA20ox1 and GA3ox2 genes in different tissues such as endosperm, embryo, coleoptile, and root. These results suggest that BR plays a role in regulating the expression of ABA and GA metabolic genes during and after seed germination. | en_US |
dc.description.note | February 2020 | en_US |
dc.identifier.uri | http://hdl.handle.net/1993/34372 | |
dc.language.iso | eng | en_US |
dc.rights | open access | en_US |
dc.subject | Seed vigor | en_US |
dc.subject | Seed germination | en_US |
dc.subject | Seedling growth | en_US |
dc.subject | Brassinosteroid | en_US |
dc.title | Regulation of wheat seed germination and seedling growth by brassinosteroid | en_US |
dc.type | master thesis | en_US |