Clostridium difficile transcriptomics and metronidazole resistance

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dc.contributor.authorZhang, Jason J.
dc.contributor.examiningcommitteeAlfa, Michelle (Medical Microbiology) de Kievit, Teri (Microbiology)en_US
dc.contributor.supervisorMulvey, Michael (Medical Microbiology)en_US
dc.date.accessioned2012-09-28T14:04:29Z
dc.date.available2012-09-28T14:04:29Z
dc.date.issued2012-09-28
dc.degree.disciplineMedical Microbiologyen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractThis is a two-part project. Proton pump inhibitors (PPIs) have been associated with increased risk of C. difficile infections and increased toxin production when combined with antimicrobial therapy. The first part of this project involved characterization of a hypervirulent NAP1 C. difficile strain, including genome sequencing and assembly, and the development of methods to study its transcriptomics using RNA-Seq, which will enable future researchers to study different expression patterns when toxigenic C. difficile is challenged with PPIs and/or antimicrobials in vitro. The second part of this project involved characterizing a clinical isolate of a NAP1 C. difficile displaying a markedly elevated MIC to metronidazole (MIC = 16 mg/mL), which initially exhibited MIC of 32 mg/mL. A method of obtaining a metronidazole-susceptible revertant from this isolate was developed and a revertant was obtained. The genomes of both isolates were sequenced, assembled, and aligned, then compared to each other for polymorphisms.en_US
dc.description.noteFebruary 2013en_US
dc.identifier.urihttp://hdl.handle.net/1993/9158
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectClostridiumen_US
dc.subjectdifficileen_US
dc.subjecttranscriptomicsen_US
dc.subjectCDIen_US
dc.subjectCDADen_US
dc.subjectmetronidazoleen_US
dc.subjectresistanceen_US
dc.subjectPPIen_US
dc.subjecttranscriptomeen_US
dc.subjectRNA-seqen_US
dc.subjectNAP1en_US
dc.subjectNAP2en_US
dc.subjectPFGEen_US
dc.subject454en_US
dc.subjectpyrosequencingen_US
dc.subjectMICen_US
dc.subjectmicrocolonyen_US
dc.subjectmicrocoloniesen_US
dc.subjectheteroresistanceen_US
dc.titleClostridium difficile transcriptomics and metronidazole resistanceen_US
dc.typemaster thesisen_US
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