Cerebellar corticogenesis in the lysosomal acid phosphatase (acp2) mutant mice: purkinje cell migration disorder
| dc.contributor.author | Ashtari, Niloufar | |
| dc.contributor.examiningcommittee | Del Bigio, Marc (Pathology) Ghia, Jean-Eric (Immunology) | en_US |
| dc.contributor.supervisor | Marzban, Hassan (Human Anatomy and Cell Science) Ghavami, Saeid (Human Anatomy and Cell Science) | en_US |
| dc.date.accessioned | 2017-05-26T20:02:02Z | |
| dc.date.available | 2017-05-26T20:02:02Z | |
| dc.date.issued | 2017 | |
| dc.degree.discipline | Human Anatomy and Cell Science | en_US |
| dc.degree.level | Master of Science (M.Sc.) | en_US |
| dc.description.abstract | In a mutant mouse called nax as the result of mutation in Lysosomal Acid phosphatase (Acp2), layers of the cerebellar cortex are impaired and monolayer Purkinje cells (Pcs) turn to multi-layered Pcs that ectopically invade the molecular layer. We investigated reelin-Dab1 signaling as an important pathway for Pcs migration and monolayer formation in cerebellum. ERK1/2 is a member of mitogen activated kinases family and suggested to be a downstream of reelin signaling. We hypothesize that the establishment of mono-layered Pcs rely on reelin through ERK1/2 pathway. Acp2 mutant mice were used for this study and molecular expression and distribution were assessed by immunohistochemistry, RT-PCR, western blotting, and cell culture. Results suggest that reelin may modulate the ERK1/2 expression, thus lower expression of reelin and higher phosphorylation of Dab1 leads to over expression of the ERK1/2 that causes the Pcs to over migrate and form multilayer in nax cerebellar cortex. | en_US |
| dc.description.note | October 2017 | en_US |
| dc.identifier.uri | http://hdl.handle.net/1993/32254 | |
| dc.language.iso | eng | en_US |
| dc.rights | open access | en_US |
| dc.subject | Cerebellum, Purkinje cell, Migration, Development, Acp2 | en_US |
| dc.title | Cerebellar corticogenesis in the lysosomal acid phosphatase (acp2) mutant mice: purkinje cell migration disorder | en_US |
| dc.type | master thesis | en_US |