Immediate and delayed suppression of type I interferon induction in porcine immune cells by Nipah virus Malaysia V protein

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dc.contributor.authorCasey, Graham
dc.contributor.examiningcommitteeBall, Blake (Medical Microbiology and Infectious Diseases) Murooka, Thomas (Immunology) Nfon, Charles (Animal Science)en_US
dc.contributor.supervisorWeingartl, Hana (Medical Microbiology and Infectious Diseases)en_US
dc.date.accessioned2019-09-04T12:30:30Z
dc.date.available2019-09-04T12:30:30Z
dc.date.issued2019-09-03en_US
dc.date.submitted2019-06-14T19:03:12Zen
dc.date.submitted2019-09-04T03:17:13Zen
dc.degree.disciplineMedical Microbiology and Infectious Diseasesen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractNipah virus Malaysia genotype (NiV-M) is an emerging zoonotic paramyxovirus. The non-structural P gene products of NiV-M (the V and W proteins) have been implicated in the block of type I interferon signaling, but live virus investigations of their roles in the porcine host are lacking. In this investigation, recombinant NiV-M that do not express either the V or W proteins were used to examine the relevance of these proteins to the induction of type I IFN in porcine immune cells. The V protein was found to be important to the production of infectious virus in porcine immune cells by limiting the production of type I IFNs both immediately after entry and later in the infectious cycle. Immunogold electron microscopy (IEM) imaging indicated that the NiV-M V and W proteins are incorporated into the NiV-M virion by attachments to the nucleocapsid. These findings show that the V protein of NiV-M suppresses type I IFN production throughout infection of porcine immune cells and demonstrate a mechanism by which NiV-M modulates the immune response in swine.en_US
dc.description.noteOctober 2019en_US
dc.identifier.urihttp://hdl.handle.net/1993/34140
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectNipah virusen_US
dc.subjectInterferon evasionen_US
dc.subjectPorcine immunityen_US
dc.titleImmediate and delayed suppression of type I interferon induction in porcine immune cells by Nipah virus Malaysia V proteinen_US
dc.typemaster thesisen_US
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