Mechanobiology regulation of airway epithelial barrier function

Simple item page
dc.contributor.authorRoy, Neilloy
dc.contributor.examiningcommitteeHalayko, Andrew (Physiology and Pathophysiology) Dakshinamurti, Shyamala (Physiology and Pathophysiology) Diehl-Jones, Bill (Biological Sciences)en_US
dc.contributor.supervisorWest, Adrian (Physiology and Pathophysiology)en_US
dc.date.accessioned2019-01-07T17:18:28Z
dc.date.available2019-01-07T17:18:28Z
dc.date.issued2018en_US
dc.date.submitted2018-12-15T04:28:12Zen
dc.degree.disciplinePhysiology and Pathophysiologyen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractEpithelial dysfunction and subepithelial fibrosis in the airways are pathophysiological features that indirectly promote airflow obstruction in asthma. Respectively, these changes result in impaired epithelial barrier function and airway stiffening. I hypothesize that subepithelial matrix stiffening disrupts airway epithelial barrier function. I engineered an experimental platform to replicate airway stiffening using lung epithelial cell lines. This platform allowed measurement of barrier function (permeability assay), in addition to traditional measurements of mRNA abundance (qPCR), protein abundance (Western blot) and protein localization (immunofluorescence microscopy). Substrate stiffness did not impact barrier-related gene and protein markers but did regulate their subcellular locations. Moreover, I observed differential responses of barrier function in response to transforming growth factor β across different substrate stiffnesses. These proof-of-concept results demonstrate substrate stiffness may regulate epithelial barrier function in a disease-relevant manner, and that my platform can be used to explore how airway stiffening regulates airway epithelial function in asthma.en_US
dc.description.noteFebruary 2019en_US
dc.identifier.urihttp://hdl.handle.net/1993/33628
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectAirwayen_US
dc.subjectCell biologyen_US
dc.subjectEpithelial permeabilityen_US
dc.subjectMechanobiologyen_US
dc.subjectMechanotransductionen_US
dc.subjectAsthmaen_US
dc.subjectLungen_US
dc.subjectRespiratoryen_US
dc.subjectExtracellular matrixen_US
dc.subjectMatrix stiffnessen_US
dc.subjectFourier transform infrared spectroscopyen_US
dc.subjectX-ray photoelectron spectroscopyen_US
dc.subjectBiomedical engineeringen_US
dc.subjectTissue engineeringen_US
dc.subjectTransforming growth factor betaen_US
dc.subjectEpithelial junctionsen_US
dc.subjectA549en_US
dc.subjectBeas-2ben_US
dc.subjectEpithelial cellen_US
dc.titleMechanobiology regulation of airway epithelial barrier functionen_US
dc.typemaster thesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Roy_Neilloy.pdf
Size:
13.54 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
2.2 KB
Format:
Item-specific license agreed to upon submission
Description:
Download
Collections
FGS - Electronic Theses and Practica