Study of the dissemination of cefoxitin-resistant Salmonella enterica serovar Heidelberg from human, abattoir poultry and retail poultry sources
| dc.contributor.author | Edirmanasinghe, Romaine Cathy Shalini | |
| dc.contributor.examiningcommittee | Wylie, John (Medical Microbiology, Community Health Sciences) Brassinga, Ann Karen (Microbiology) | en_US |
| dc.contributor.supervisor | Mulvey, Michael (Medical Microbiology) Golding, George (Medical Microbiology) | en_US |
| dc.date.accessioned | 2016-09-15T14:30:54Z | |
| dc.date.available | 2016-09-15T14:30:54Z | |
| dc.date.issued | 2016 | |
| dc.degree.discipline | Medical Microbiology | en_US |
| dc.degree.level | Master of Science (M.Sc.) | en_US |
| dc.description.abstract | This study characterized Salmonella enterica serovar Heidelberg from human, abattoir poultry and retail poultry isolates to examine the molecular relationships of cefoxitin resistance between these groups. A total of 147 S. Heidelberg (70 cefoxitin-resistant and 77 cefoxitin-susceptible) isolates were studied. All cefoxitin-resistant isolates were also resistant to amoxicillin-clavulanic acid, ampicillin, ceftiofur and ceftriaxone, and all contained the CMY-2 gene. Pulsed-field gel electrophoresis typing illustrated that 93.9% isolates clustered together with ≥ 90% similarity. Core genome analysis using whole genome sequencing identified 12 clusters of isolates with zero to four single nucleotide variations. These clusters consisted of cefoxitin-resistant and susceptible human, abattoir poultry and retail poultry isolates. Analysis of CMY-2 plasmids from cefoxitin-resistant isolates revealed all belonged to incompatibility group I1. Analysis of plasmid sequences using WGS revealed high identity (95-99%) to a previously described plasmid (pCVM29188_101) found in Salmonella Kentucky. When compared to pCVM29188_101, all sequenced cefoxitin-resistant isolates were found to carry one of ten possible variant plasmids. The discovery of several clusters of isolates from different sources with zero to four SNVs suggests that transmission between human, abattoir poultry and retail poultry sources may be occurring. The classification of newly sequenced plasmids into one of ten sequence variant types suggests transmission of a common CMY-2 plasmid amongst S. Heidelberg with variable genetic backgrounds. | en_US |
| dc.description.note | October 2016 | en_US |
| dc.identifier.uri | http://hdl.handle.net/1993/31772 | |
| dc.language.iso | eng | en_US |
| dc.rights | open access | en_US |
| dc.subject | Antibiotic resistance | en_US |
| dc.subject | Beta-lactamases | en_US |
| dc.subject | Cefoxitin | en_US |
| dc.subject | Salmonella enterica serovar Heidelberg | en_US |
| dc.subject | Whole genome sequencing | en_US |
| dc.title | Study of the dissemination of cefoxitin-resistant Salmonella enterica serovar Heidelberg from human, abattoir poultry and retail poultry sources | en_US |
| dc.type | master thesis | en_US |