Seed-specific expression of the class 2 Phytoglobin (Pgb2) increases seed oil in Brassica napus L.

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dc.contributor.authorHaq, Md Ehsanul
dc.contributor.examiningcommitteeDuncan, Robert W. (Plant Science)
dc.contributor.examiningcommitteeHill, Robert D. (Plant Science)
dc.contributor.examiningcommitteeRenault, Sylvie (Biological Sciences)
dc.contributor.supervisorStasolla, Claudio
dc.date.accessioned2023-08-31T15:40:00Z
dc.date.available2023-08-31T15:40:00Z
dc.date.issued2023-05-10
dc.date.submitted2023-06-16T14:20:38Zen_US
dc.degree.disciplinePlant Scienceen_US
dc.degree.levelMaster of Science (M.Sc.)
dc.description.abstractTo examine the function of phytoglobin 2 (Pgb2) on seed oil level in the oil-producing crop Brassica napus L., we generated transgenic plants in which BnPgb2 was over-expressed in the seeds using the cruciferin1 promoter. Over-expression of BnPgb2 elevated the amount of oil, which showed a positive relationship with the level of BnPgb2, without altering the oil nutritional value, as evidenced by the lack of major changes in composition of fatty acids (FA), and key agronomic traits. Two key transcription factors, LEAFY COTYLEDON1 (LEC1) and WRINKLED1 (WRI1), known to promote the synthesis of fatty acids (FA) and potentiate oil accumulation, were induced in BnPgb2 over-expressing seeds. The concomitant induction of several enzymes of sucrose metabolism, SUCROSE SYNTHASE1 (SUS) 1 and 3, FRUCTOSE BISPHOSPHATE ALDOLASE (FPA), and PHOSPHOGLYCERATE KINASE (PGK), and starch synthesis, ADP-GLUCOSE PHOSPHORYLASE (AGPase) suggests that BnPgb2 favors sugar mobilization for FA production. The two plastid FA biosynthetic enzymes SUBUNIT A OF ACETYL-CoA CARBOXYLASE (ACCA2), and MALONYL-CoA:ACP TRANSACYLASE (MCAT) were also up-regulated by the over-expression of BnPgb2. The requirement of BnPgb2 for oil deposition was further evidenced in natural germplasm by the higher levels of BnPgb2 in seeds of high-oil genotypes relative to their low-oil counterparts.
dc.description.noteOctober 2023
dc.identifier.urihttp://hdl.handle.net/1993/37527
dc.language.isoeng
dc.rightsopen accessen_US
dc.subjectPhytoglobin 2
dc.subjectSeed oil
dc.subjectFatty acids
dc.titleSeed-specific expression of the class 2 Phytoglobin (Pgb2) increases seed oil in Brassica napus L.
dc.typemaster thesisen_US
local.subject.manitobano
project.funder.identifierhttps://doi.org/10.13039/100010318
project.funder.nameUniversity of Manitoba
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