The attenuated virulence of a Burkholderia cenocepacia K56-2 paaABCDE mutant is due to inhibition of quorum sensing by release of phenylacetic acid

Simple item page
dc.contributor.authorPribytkova, Tatiana
dc.contributor.examiningcommitteeBrassinga, Ann Karen (Microbiology) Sorensen, John L. (Chemistry)en_US
dc.contributor.supervisorCardona, Silvia T.(Microbiology)en_US
dc.date.accessioned2014-09-03T22:52:33Z
dc.date.available2014-09-03T22:52:33Z
dc.date.issued2014-09-03
dc.degree.disciplineMicrobiologyen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractThe phenylacetic acid degradation pathway of Burkholderia cenocepacia is necessary for full pathogenicity of B. cenocepacia in nematode; however, the reasons of such requirements are unknown. Unlike wild type B. cenocepacia, a deletion mutant of the phenylacetyl-CoA monooxygenase complex (ΔpaaABCDE) released phenylacetic acid extracellularly in conditions that allow infection in Caenorhabditis elegans. Addition of phenylacetic acid further decreased the pathogenicity of the ΔpaaABCDE, which cannot metabolize phenylacetic acid, but did not affect the wild type, due to phenylacetic acid consumption. Detection of acyl-homoserine lactones was reduced in spent medium from ΔpaaABCDE compared to that of the wild type strain. Phenotypes repressed in ΔpaaABCDE, protease activity and pathogenicity against C. elegans, increased with the addition of exogenous N-octanoyl-L-homoserine lactone. Thus, it was demonstrated that the attenuated phenotype of B. cenocepacia ΔpaaABCDE is due to quorum sensing inhibition by release of phenylacetic acid, affecting N-octanoyl-L-homoserine lactone signaling.en_US
dc.description.noteOctober 2014en_US
dc.identifier.urihttp://hdl.handle.net/1993/23961
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectBurkholderiaen_US
dc.subjectphenylacetic aciden_US
dc.subjectQuorum sensingen_US
dc.titleThe attenuated virulence of a Burkholderia cenocepacia K56-2 paaABCDE mutant is due to inhibition of quorum sensing by release of phenylacetic aciden_US
dc.typemaster thesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Pribytkova_Tatiana.pdf
Size:
1.92 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
2.25 KB
Format:
Item-specific license agreed to upon submission
Description:
Download
Collections
FGS - Electronic Theses and Practica