Attenuation of Oxidative Stress of Erythrocytes by Plant-Derived Flavonoids, Orientin and Luteolin

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dc.contributor.authorAn, Fang
dc.contributor.authorWang, Shulin
dc.contributor.authorYuan, Danhua
dc.contributor.authorGong, Yuewen
dc.contributor.authorWang, Shuhua
dc.date.accessioned2016-05-11T14:27:50Z
dc.date.available2016-05-11T14:27:50Z
dc.date.issued2016-2-4
dc.date.updated2016-05-08T09:31:16Z
dc.description.abstractErythrocytes are easy to be injured by oxidative stress in their lifespan. Although there are several chemicals such as vitamin C (VC) that would be able to reduce oxidative stress, natural herbal products still remain an interesting research area. The current study investigated the effects of two plant-derived flavonoids, orientin and luteolin, on erythrocytes and their possible mechanisms. This experiment was divided into nine groups, which were normal group, model group, VC control group, and treated groups with different doses of orientin and luteolin (10, 20, and 40 μg/mL), respectively. Hemolysis rate was determined by spectrophotometry. Antioxidative enzyme and products were evaluated by different methods. Erythrocyte cell surface and cellular structure were observed with scanning or transmission electron microscope, respectively. Oxidative stress induced significant increase in hemolysis rate of erythrocytes. Orientin or luteolin ameliorated hemolysis of erythrocytes in oxidative stress in a dose-dependent manner. Both orientin and luteolin reduced oxidative products and increased antioxidative enzyme activities. Moreover, orientin and luteolin attenuated oxidative stress induced damage of erythrocyte cell surface morphology and cellular structure. In conclusion, orientin and luteolin could protect human erythrocytes from oxidative damage by attenuating oxidative stress, protecting antioxidative enzyme activities, and preserving integrity of erythrocyte structure.
dc.description.versionPeer Reviewed
dc.identifier.citationFang An, Shulin Wang, Danhua Yuan, Yuewen Gong, and Shuhua Wang, “Attenuation of Oxidative Stress of Erythrocytes by Plant-Derived Flavonoids, Orientin and Luteolin,” Evidence-Based Complementary and Alternative Medicine, vol. 2016, Article ID 3401269, 8 pages, 2016. doi:10.1155/2016/3401269
dc.identifier.urihttp://dx.doi.org/10.1155/2016/3401269
dc.identifier.urihttp://hdl.handle.net/1993/31286
dc.language.rfc3066en
dc.rightsopen accessen_US
dc.rights.holderCopyright © 2016 Fang An et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.titleAttenuation of Oxidative Stress of Erythrocytes by Plant-Derived Flavonoids, Orientin and Luteolin
dc.typeJournal Article
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