A comparative characterization of methyl-CpG binding protein 2 isoforms in the developing and adult mouse brain

dc.contributor.authorEzeonwuka, Chinelo Dorris
dc.contributor.examiningcommitteeSimard, Louise (Biochemistry and Medical Genetics) Kong, Jiming (Human Anatomy and Cell Science)en_US
dc.contributor.supervisorMojgan, Rastegar (Biochemistry and Medical Genetics)en_US
dc.date.accessioned2013-08-16T15:02:26Z
dc.date.available2013-08-16T15:02:26Z
dc.date.issued2013-08-16
dc.degree.disciplineBiochemistry and Medical Geneticsen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractMethyl-CpG binding protein 2 (MeCP2) is an epigenetic regulator in brain that binds to both methylated and hyroxymethylated DNA with similar affinities. MeCP2 has two isoforms, MeCP2E1 and MeCP2E2 with distinct N-terminus. These isoforms are generated by alternative splicing of the MECP2/Mecp2 gene. Despite the wealth of knowledge about the molecular properties of MeCP2 as a whole protein, little is known regarding its isoform components. Importantly, basic fundamental questions such as MeCP2 isoform-specific expression profile and the underlying regulatory mechanisms are yet to be fully addressed. To further explore the role(s) of MeCP2 isoforms, we have characterized MeCP2 isoform-specific expression profiles. In this study, we show that MeCP2 isoforms are differentially distributed in the adult murine brain, and are temporally expressed during mouse brain development. Our study allows us to gain further insight into the function(s) of MeCP2 isoforms in the central nervous system.en_US
dc.description.noteOctober 2013en_US
dc.identifier.urihttp://hdl.handle.net/1993/22028
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectMeCP2en_US
dc.subjectExpressionen_US
dc.titleA comparative characterization of methyl-CpG binding protein 2 isoforms in the developing and adult mouse brainen_US
dc.typemaster thesisen_US
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