Stress hormone regulation of splicing in the hippocampus

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dc.contributor.authorOlude, Joseph
dc.contributor.examiningcommitteeYao, Xiao-Jian (Medical Microbiology and Infectious Diseases)en_US
dc.contributor.examiningcommitteeSiddiqui, Tabrez (Physiology and Pathophysiology)en_US
dc.contributor.supervisorXie, Jiuyong (Physiology and Pathophysiology)en_US
dc.date.accessioned2020-08-24T19:12:55Z
dc.date.available2020-08-24T19:12:55Z
dc.date.copyright2020-08-18
dc.date.issued2020-08en_US
dc.date.submitted2020-08-14T17:23:11Zen_US
dc.date.submitted2020-08-18T17:13:14Zen_US
dc.degree.disciplinePhysiology and Pathophysiologyen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractOBJECTIVES: The regulation of splicing by glucocorticoids in the hippocampus has not been well studied. Hence, this study aims to determine the transcripts/exons that are regulated by stress hormones in the hippocampus. RESULTS: Hypophysectomy was confirmed by weight reduction in experimental animals. Serum corticosterone was also reduced upon hypophysectomy which was rescued by administration of CORT or ACTH. Using RNA-Seq, we identified transcripts/exons that are changed upon hypophysectomy and successfully rescued by CORT or ACTH. Specifically, edgeR and DEXSeq identified 32 genes with >2 fold changes and 889 exons with >1.5 fold changes respectively that were changed upon hypophysectomy and rescued by CORT or ACTH administration. RT-PCR validated the regulation of several target exons by stress hormones. We further identified a decline and an increase in the transcripts containing the target exon of the Sept8 gene in a time-course dependent manner upon hypophysectomy and in normal groups respectively. CONCLUSION: These findings demonstrate a specific exon that is regulated by stress hormones in the hippocampus.en_US
dc.description.noteOctober 2020en_US
dc.identifier.urihttp://hdl.handle.net/1993/34886
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectStress hormonesen_US
dc.subjectGlucocorticoidsen_US
dc.subjectAlternative splicingen_US
dc.subjectHippocampusen_US
dc.subjectRNA-Seqen_US
dc.titleStress hormone regulation of splicing in the hippocampusen_US
dc.typemaster thesisen_US
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