Blood monocyte and dendritic cell profiles among people living with HIV with Mycobacterium tuberculosis co-infection

Simple item page
dc.contributor.authorRambaran, Santhuri
dc.contributor.authorMaseko, Thando G.
dc.contributor.authorLewis, Lara
dc.contributor.authorHassan-Moosa, Razia
dc.contributor.authorArchary, Derseree
dc.contributor.authorNgcapu, Sinaye
dc.contributor.authorGarrett, Nigel
dc.contributor.authorMcKinnon, Lyle R.
dc.contributor.authorpadayatchi, nesri
dc.contributor.authorNaidoo, Kogieleum
dc.contributor.authorSivro, Aida
dc.date.accessioned2023-11-14T20:19:44Z
dc.date.available2023-11-14T20:19:44Z
dc.date.issued2023-07-21
dc.date.updated2023-11-03T09:16:00Z
dc.description.abstractBackground Understanding the complex interactions of the immune response mediated by Mycobacterium tuberculosis and HIV co-infection is fundamental to disease biomarker discovery, vaccine, and drug development. Using flow cytometry, we characterized the frequencies and phenotypic differences in monocytes and dendritic cell populations using peripheral blood mononuclear cells from individuals with recurrent, active pulmonary tuberculosis with and without coexisting HIV infection (CAPRISA 011, Clinicaltrials.gov, NCT02114684, 29/01/2014) and compared them to samples from HIV positive individuals and healthy controls. Additionally, we assessed the associations between the frequency of monocyte and dendritic cell subsets and time to culture conversion and cavitary disease in patients with active TB using a cox proportional hazards and logistic regression models. Results Compared to healthy controls, the frequency of total monocytes (HLA-DR + CD14 +) was significantly higher in the TB/HIV and TB groups and the frequency of dendritic cells (HLA-DR + CD14-) was significantly higher in TB/HIV and HIV groups. We observed significant variation in the expression of CCR2, CD40, CD11b, CD86, CD163, CX3CR1 across different cell subsets in the four study groups. Increase in CCR2, CD11b and CD40 was associated with active TB infection, while decrease in CX3CR1 and increase in CD163 was associated with HIV infection. Expression of CX3CR1 (aHR 0.98, 95% CI 0.963 – 0.997, p = 0.019) on non-classical monocytes associated with longer time to TB culture conversion in the multivariable model correcting for randomization arm, age, sex, HIV status, lung cavitation, alcohol use, smoking and BMI. Higher surface expression of CD86 (aOR 1.017, 95% CI 1.001 – 1.032, p = 0.033) on intermediate monocytes associated with the presence of lung cavitation, while higher expression of transitional monocytes (aOR 0.944, 95% CI 0.892 – 0.999, p = 0.047) associated with the absence of lung cavitation in the multivariable model. Conclusion These data provide valuable insight into the heterogenous role of monocyte and dendritic cells in TB and HIV infections.
dc.identifier.citationBMC Immunology. 2023 Jul 21;24(1):21
dc.identifier.doi10.1186/s12865-023-00558-z
dc.identifier.urihttp://hdl.handle.net/1993/37787
dc.language.isoeng
dc.language.rfc3066en
dc.publisherBMC
dc.rightsopen accessen_US
dc.rights.holderThe Author(s)
dc.subjectPulmonary‑tuberculosis
dc.subjectInflammation
dc.subjecthuman immunodeficiency virus
dc.subjectmonocytes
dc.subjectdendritic cells
dc.titleBlood monocyte and dendritic cell profiles among people living with HIV with Mycobacterium tuberculosis co-infection
dc.typeJournal Article
local.author.affiliationRady Faculty of Health Sciences::Max Rady College of Medicine::Department of Medical Microbiology and Infectious Diseases
oaire.awardTitleStrategic Health Innovation Partnerships (SHIP)
oaire.citation.issue21
oaire.citation.titleBMC Immunology
oaire.citation.volume24
project.funder.identifierhttp://dx.doi.org/10.13039/501100001322
project.funder.nameSouth African Medical Research Council
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Article
Size:
1.74 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
770 B
Format:
Item-specific license agreed to upon submission
Description:
Download
Collections
University of Manitoba Scholarship
Rady Faculty of Health Sciences Scholarly Works