Pseudomonas chlororaphis PA23 biocontrol of Sclerotinia sclerotiorum on canola: understanding populations and enhancing inoculation

Simple item page
dc.contributor.authorReimer, Lori
dc.contributor.examiningcommitteeDuncan, Robert (Plant Science) De Kievit, Teresa (Micorbiology) Belmonte, Mark (Biological Sciences)en_US
dc.contributor.supervisorFernando, Dilantha (Plant Science)en_US
dc.date.accessioned2016-10-14T21:01:40Z
dc.date.available2016-10-14T21:01:40Z
dc.date.issued2016
dc.degree.disciplinePlant Scienceen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractPseudomonas chlororaphis strain PA23 has demonstrated biocontrol of Sclerotinia sclerotiorum (Lib.) de Bary, a fungal pathogen of canola (Brassica napus L.). The objectives of this research were two-fold: to optimize PA23 phyllosphere biocontrol and to investigate PA23’s influence in the rhizosphere. PA23 demonstrated longevity when inoculated on B. napus under greenhouse conditions. Carbon source differentially effected growth rate and antifungal metabolite production of PA23 in culture. Carbon source did not have a significant effect on in vivo biocontrol. PA23 demonstrated biocontrol ability of the fungal root pathogens Rhizoctonia solani J.G. Kühn and Pythium ultimum Trow in radial diffusion assays. PA23’s ability to promote seedling root growth was demonstrated in sterile growth pouches, but in a soil system these results were reversed. This research is essential for developing PA23 into an effective biocontrol agent in the phyllosphere and it opens the door for use of PA23 as a rhizosphere seed treatment.en_US
dc.description.noteOctober 2016en_US
dc.identifier.urihttp://hdl.handle.net/1993/31890
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectPseudomonas chlororaphis PA23en_US
dc.subjectSclerotinia sclerotiorumen_US
dc.subjectcanolaen_US
dc.titlePseudomonas chlororaphis PA23 biocontrol of Sclerotinia sclerotiorum on canola: understanding populations and enhancing inoculationen_US
dc.typemaster thesisen_US
Files
Original bundle
Now showing 1 - 5 of 10
Thumbnail Image
Name:
lori_reimer.pdf
Size:
1.38 MB
Format:
Adobe Portable Document Format
Description:
Thesis
Download
Thumbnail Image
Name:
figure1.1_rightslink_printable_license.pdf
Size:
96.28 KB
Format:
Adobe Portable Document Format
Description:
Download
Thumbnail Image
Name:
figure1.2_rightslink_printable_license.pdf
Size:
240.7 KB
Format:
Adobe Portable Document Format
Description:
Download
Thumbnail Image
Name:
figure1.3_rightslink_printable_license.pdf
Size:
123.43 KB
Format:
Adobe Portable Document Format
Description:
Download
Thumbnail Image
Name:
figure1.4_permission_from_asm.pdf
Size:
261.26 KB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
2.2 KB
Format:
Item-specific license agreed to upon submission
Description:
Download
Collections
FGS - Electronic Theses and Practica