Mucosal antibody responses to vaccines targeting SIV protease cleavage sites or full-length Gag and Env proteins in Mauritian cynomolgus macaques

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dc.contributor.authorLi, Hongzhao
dc.contributor.authorHai, Yan
dc.contributor.authorLim, So-Yon
dc.contributor.authorToledo, Nikki
dc.contributor.authorCrecente-Campo, Jose
dc.contributor.authorSchalk, Dane
dc.contributor.authorLi, Lin
dc.contributor.authorOmange, Robert
dc.contributor.authorDacoba, Tamara
dc.contributor.authorLiu, Lewis
dc.contributor.authorKashem, Mohammad
dc.contributor.authorWan, Yanmin
dc.contributor.authorLiang, Binhua
dc.contributor.authorLi, Qingsheng
dc.contributor.authorRakasz, Eva
dc.contributor.authorSchultz-Darken, Nancy
dc.contributor.authorAlonso, Maria
dc.contributor.authorPlummer, Francis
dc.contributor.authorWhitney, James
dc.contributor.authorLuo, Ma
dc.date.accessioned2021-08-12T16:45:06Z
dc.date.available2021-08-12T16:45:06Z
dc.date.issued2018-08-28
dc.date.submitted2021-08-12T02:09:19Zen_US
dc.description.abstractHIV mutates rapidly and infects CD4+ T cells, especially when they are activated. A vaccine targeting conserved, essential viral elements while limiting CD4+ T cell activation could be effective. Learning from natural immunity observed in a group of highly HIV-1 exposed seronegative Kenyan female sex workers, we are testing a novel candidate HIV vaccine targeting the 12 viral protease cleavage sites (PCSs) (the PCS vaccine), in comparison with a vaccine targeting full-length Gag and Env (the Gag/Env vaccine) in a Mauritian cynomolgus macaque/SIV model. In this study, we evaluated these vaccines for induction of mucosal antibodies to SIV immunogens at the female genital tract. Bio-Plex and Western blot analyses of cervicovaginal lavage samples showed that both the PCS and Gag/Env vaccines can elicit mucosal IgG antibody responses to SIV immunogens. A significantly higher increase of anti-PCS antibodies was induced by the PCS vaccine than by the Gag/Env vaccine (p<0.0001). The effect of the mucosal antibody responses in protection from repeated low dose pathogenic SIVmac251 challenges is being evaluated.en_US
dc.description.sponsorshipThis study was supported by the following funding awarded to ML: a National Institute of Allergy and Infectious Diseases grant, with grant number: R01AI111805 and URL: https:// www.niaid.nih.gov/; a Canadian Institutes of Health Research/Canadian HIV Vaccine Initiative Bridging grant (no grant number available), with URL: http:// www.cihr-irsc.gc.ca/e/42458.html; and funding from National Microbiology Laboratory of Canada (no grant number available), with URL: https://www.nml-lnm.gc.ca/index-eng.htm. Research reported in this publication was supported in part by the Office of the Director, National Institutes of Health under Award Number P51OD011106 to the Wisconsin National Primate Research Center, University of Wisconsin-Madison. This research was conducted in part at a facility constructed with support from Research Facilities Improvement Program grant numbers RR15459-01 and RR020141-01. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en_US
dc.identifier.citationLi H, Hai Y, Lim S-Y, Toledo N, Crecente- Campo J, Schalk D, et al. (2018) Mucosal antibody responses to vaccines targeting SIV protease cleavage sites or full-length Gag and Env proteins in Mauritian cynomolgus macaques. PLoS ONE 13 (8): e0202997. https://doi.org/10.1371/journal.pone.0202997en_US
dc.identifier.doi10.1371/journal.pone.0202997
dc.identifier.urihttp://hdl.handle.net/1993/35798
dc.language.isoengen_US
dc.publisherPLOSen_US
dc.rightsopen accessen_US
dc.subjectMucosal antibodyen_US
dc.subjectProtease cleavage sitesen_US
dc.subjectVaccineen_US
dc.subjectFull-length Gag and Enven_US
dc.subjectMauritian cynomolgus macaquesen_US
dc.subjectMonkeysen_US
dc.titleMucosal antibody responses to vaccines targeting SIV protease cleavage sites or full-length Gag and Env proteins in Mauritian cynomolgus macaquesen_US
dc.typeArticleen_US
local.author.affiliationRady Faculty of Health Sciencesen_US
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